Abstract
By genetic complementation in a yeast choline transport mutant from a yeast gene library, we isolated plasmids encoding choline transport. The cloned plasmids contained a common 4.0-kilobase DNA fragment and also complemented an ethanolamine transport defect. The cloned sequence present in the yeast genome was possibly unique.
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- Beggs J. D. Transformation of yeast by a replicating hybrid plasmid. Nature. 1978 Sep 14;275(5676):104–109. doi: 10.1038/275104a0. [DOI] [PubMed] [Google Scholar]
- Broach J. R., Strathern J. N., Hicks J. B. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene. 1979 Dec;8(1):121–133. doi: 10.1016/0378-1119(79)90012-x. [DOI] [PubMed] [Google Scholar]
- Hosaka K., Yamashita S. Choline transport in Saccharomyces cerevisiae. J Bacteriol. 1980 Jul;143(1):176–181. doi: 10.1128/jb.143.1.176-181.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hosaka K., Yamashita S. Induction of choline transport and its role in the stimulation of the incorporation of choline into phosphatidylcholine by polyamines in a polyamine auxotroph of Saccharomyces cerevisiae. Eur J Biochem. 1981 May;116(1):1–6. doi: 10.1111/j.1432-1033.1981.tb05292.x. [DOI] [PubMed] [Google Scholar]
- Katsu K., Inoue M., Mitsuhashi S. Transposition of the carbenicillin-hydrolyzing beta-lactamase gene. J Bacteriol. 1982 May;150(2):483–489. doi: 10.1128/jb.150.2.483-489.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nikawa J., Nagumo T., Yamashita S. Myo-inositol transport in Saccharomyces cerevisiae. J Bacteriol. 1982 May;150(2):441–446. doi: 10.1128/jb.150.2.441-446.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nikawa J., Yamashita S. 2-hydroxyethylhydrazine as a potent inhibitor of phospholipid methylation in yeast. Biochim Biophys Acta. 1983 Apr 13;751(2):201–209. [PubMed] [Google Scholar]
- Nikawa J., Yamashita S. Molecular cloning of the gene encoding CDPdiacylglycerol-inositol 3-phosphatidyl transferase in Saccharomyces cerevisiae. Eur J Biochem. 1984 Sep 3;143(2):251–256. doi: 10.1111/j.1432-1033.1984.tb08366.x. [DOI] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Steiner M. R., Lester R. L. In vitro studies of phospholipid biosynthesis in Saccharomyces cerevisiae. Biochim Biophys Acta. 1972 Feb 21;260(2):222–243. doi: 10.1016/0005-2760(72)90035-5. [DOI] [PubMed] [Google Scholar]
- Yamashita S., Oshima A., Nikawa J., Hosaka K. Regulation of the phosphatidylethanolamine methylation pathway in Saccharomyces cerevisiae. Eur J Biochem. 1982 Nov 15;128(2-3):589–595. doi: 10.1111/j.1432-1033.1982.tb07005.x. [DOI] [PubMed] [Google Scholar]
- Yamashita S., Oshima A. Regulation of phosphatidylethanolamine methyltransferase level by myo-inositol in Saccaromyces cerevisiae. Eur J Biochem. 1980 Mar;104(2):611–616. doi: 10.1111/j.1432-1033.1980.tb04465.x. [DOI] [PubMed] [Google Scholar]