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. 1986 May;166(2):368–374. doi: 10.1128/jb.166.2.368-374.1986

Nucleotide sequences of the R1-19 plasmid transfer genes traM, finP, traJ, and traY and the traYZ promoter.

B B Finlay, L S Frost, W Paranchych
PMCID: PMC214613  PMID: 3009392

Abstract

The complete nucleotide sequences of the R1 drd-19 (R1-19) plasmid transfer genes traM, finP, traJ, and traY and the region encoding the traYZ promoter were determined. The traM protein from R1-19 was similar to the 127-amino-acid traM product from the conjugative plasmid F; only 28 residues were not identical. finP, a negative regulatory element of the traJ gene, contained a 12-base-pair inverted repeat identical to that found in the F plasmid, but differed in the 7 base pairs found between the repeats. The traJ gene and the traYZ promoter (the site of transcriptional stimulation by the traJ product) were completely different from the equivalent sequences in plasmid F. Galactokinase fusion studies of the traYZ promoter indicated that the R1-19 and F plasmids have analogous but not homologous traYZ promoter strengths and regulation. The traY protein from R1-19 was 44 residues shorter than the traY product from plasmid F, but there was some homology within the C-terminal halves of the traY gene products. The predicted translational start codon for the traY gene is GUG.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Manning P. A., Edelbluth C., Herrlich P. Export without proteolytic processing of inner and outer membrane proteins encoded by F sex factor tra cistrons in Escherichia coli minicells. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4837–4841. doi: 10.1073/pnas.76.10.4837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alfaro G., Willetts N. The relationship between the transfer systems of some bacterial plasmids. Genet Res. 1972 Dec;20(3):279–289. doi: 10.1017/s0016672300013811. [DOI] [PubMed] [Google Scholar]
  3. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chou P. Y., Fasman G. D. Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978;47:45–148. doi: 10.1002/9780470122921.ch2. [DOI] [PubMed] [Google Scholar]
  6. Everett R., Willetts N. Characterisation of an in vivo system for nicking at the origin of conjugal DNA transfer of the sex factor F. J Mol Biol. 1980 Jan 15;136(2):129–150. doi: 10.1016/0022-2836(80)90309-5. [DOI] [PubMed] [Google Scholar]
  7. Finlay B. B., Frost L. S., Paranchych W. Localization, cloning, and sequence determination of the conjugative plasmid ColB2 pilin gene. J Bacteriol. 1984 Oct;160(1):402–407. doi: 10.1128/jb.160.1.402-407.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fowler T., Taylor L., Thompson R. The control region of the F plasmid transfer operon: DNA sequence of the traJ and traY genes and characterisation of the traY leads to Z promoter. Gene. 1983 Dec;26(1):79–89. doi: 10.1016/0378-1119(83)90038-0. [DOI] [PubMed] [Google Scholar]
  9. Frost L. S., Finlay B. B., Opgenorth A., Paranchych W., Lee J. S. Characterization and sequence analysis of pilin from F-like plasmids. J Bacteriol. 1985 Dec;164(3):1238–1247. doi: 10.1128/jb.164.3.1238-1247.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Frost L. S., Paranchych W., Willetts N. S. DNA sequence of the F traALE region that includes the gene for F pilin. J Bacteriol. 1984 Oct;160(1):395–401. doi: 10.1128/jb.160.1.395-401.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gaffney D., Skurray R., Willetts N. Regulation of the F conjugation genes studied by hybridization and tra-lacZ fusion. J Mol Biol. 1983 Jul 25;168(1):103–122. doi: 10.1016/s0022-2836(83)80325-8. [DOI] [PubMed] [Google Scholar]
  12. Hawley D. K., McClure W. R. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983 Apr 25;11(8):2237–2255. doi: 10.1093/nar/11.8.2237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hopp T. P., Woods K. R. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824–3828. doi: 10.1073/pnas.78.6.3824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Humphreys G. O., Willshaw G. A., Anderson E. S. A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975 Apr 2;383(4):457–463. doi: 10.1016/0005-2787(75)90318-4. [DOI] [PubMed] [Google Scholar]
  15. Inouye S., Soberon X., Franceschini T., Nakamura K., Itakura K., Inouye M. Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3438–3441. doi: 10.1073/pnas.79.11.3438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Koronakis V. E., Bauer E., Högenauer G. The traM gene of the resistance plasmid R1: comparison with the corresponding sequence of the Escherichia coli F factor. Gene. 1985;36(1-2):79–86. doi: 10.1016/0378-1119(85)90071-x. [DOI] [PubMed] [Google Scholar]
  17. Meynell E., Datta N. Mutant drug resistant factors of high transmissibility. Nature. 1967 May 27;214(5091):885–887. doi: 10.1038/214885a0. [DOI] [PubMed] [Google Scholar]
  18. Ohlendorf D. H., Anderson W. F., Lewis M., Pabo C. O., Matthews B. W. Comparison of the structures of cro and lambda repressor proteins from bacteriophage lambda. J Mol Biol. 1983 Sep 25;169(3):757–769. doi: 10.1016/s0022-2836(83)80169-7. [DOI] [PubMed] [Google Scholar]
  19. Ostermann E., Kricek F., Högenauer G. Cloning the origin of transfer region of the resistance plasmid R1. EMBO J. 1984 Aug;3(8):1731–1735. doi: 10.1002/j.1460-2075.1984.tb02039.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Raibaud O., Schwartz M. Positive control of transcription initiation in bacteria. Annu Rev Genet. 1984;18:173–206. doi: 10.1146/annurev.ge.18.120184.001133. [DOI] [PubMed] [Google Scholar]
  21. Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. doi: 10.1146/annurev.ge.13.120179.001535. [DOI] [PubMed] [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Scott J. R. Regulation of plasmid replication. Microbiol Rev. 1984 Mar;48(1):1–23. doi: 10.1016/b978-0-12-048850-6.50006-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sharp P. A., Cohen S. N., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol. 1973 Apr 5;75(2):235–255. doi: 10.1016/0022-2836(73)90018-1. [DOI] [PubMed] [Google Scholar]
  25. Shine J., Dalgarno L. Determinant of cistron specificity in bacterial ribosomes. Nature. 1975 Mar 6;254(5495):34–38. doi: 10.1038/254034a0. [DOI] [PubMed] [Google Scholar]
  26. Skurray R. A., Nagaishi H., Clark A. J. Molecular cloning of DNA from F sex factor of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1976 Jan;73(1):64–68. doi: 10.1073/pnas.73.1.64. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Thompson R., Taylor L., Kelly K., Everett R., Willetts N. The F plasmid origin of transfer: DNA sequence of wild-type and mutant origins and location of origin-specific nicks. EMBO J. 1984 May;3(5):1175–1180. doi: 10.1002/j.1460-2075.1984.tb01947.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Thompson R., Taylor L. Promoter mapping and DNA sequencing of the F plasmid transfer genes traM and traJ. Mol Gen Genet. 1982;188(3):513–518. doi: 10.1007/BF00330058. [DOI] [PubMed] [Google Scholar]
  29. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  30. Willetts N., Skurray R. The conjugation system of F-like plasmids. Annu Rev Genet. 1980;14:41–76. doi: 10.1146/annurev.ge.14.120180.000353. [DOI] [PubMed] [Google Scholar]
  31. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

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