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. 1984 Oct;160(1):15–21. doi: 10.1128/jb.160.1.15-21.1984

Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation.

M Y Yang, E Ferrari, D J Henner
PMCID: PMC214674  PMID: 6090407

Abstract

The neutral protease gene of Bacillus subtilis has been cloned, and its nucleotide sequence has been determined. The cloned gene was used to create an in vitro-derived deletion mutation, which was used to replace the wild-type copy of the gene. This deletion, in combination with a deletion of the alkaline protease gene, completely abolished protease production. The loss of the proteases had no detectable effect on growth, morphology, or sporulation.

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Selected References

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