Abstract
Batch-grown Methanobacterium thermoautotrophicum cells grew nonexponentially in the absence of exogenous Pi until intracellular cyclic-2,3-diphosphoglycerate (cyclic DPG) had fallen below 2 mumol/g (dry weight), the limit of detection. Growth resumed immediately upon transfer to medium containing Pi Cyclic DPG levels were also below detection in Pi-limited chemostat cultures operating at a dilution rate of 0.173 h-1 (4-h doubling time), with reservoir Pi concentrations below 200 microM. At this dilution rate, the Pi concentration in the culture was 4 microM. An H2-limited steady state was achieved with 400 microM Pi in the inflowing medium (67 microM in the culture). The cyclic DPG content of these cells was 72 to 74 mumol/g, about one-third the amount in batch-grown cells. The specific growth rate accelerated immediately to 0.36 h-1 (1.9-h doubling time) under washout conditions at high dilution rate. The cellular content of cyclic DPG declined over a 2-h period, and then increased rapidly as the Pi level in the medium approached 200 microM. Expansion of the cyclic DPG pool coincided with a marked increase in Pi assimilation. These results indicated that M. thermoautotrophicum accumulated cyclic DPG only when Pi and H2 were readily available.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Balch W. E., Wolfe R. S. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. Appl Environ Microbiol. 1976 Dec;32(6):781–791. doi: 10.1128/aem.32.6.781-791.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dawes E. A., Senior P. J. The role and regulation of energy reserve polymers in micro-organisms. Adv Microb Physiol. 1973;10:135–266. doi: 10.1016/s0065-2911(08)60088-0. [DOI] [PubMed] [Google Scholar]
- Kanodia S., Roberts M. F. Methanophosphagen: Unique cyclic pyrophosphate isolated from Methanobacterium thermoautotrophicum. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5217–5221. doi: 10.1073/pnas.80.17.5217. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kenealy W. R., Thompson T. E., Schubert K. R., Zeikus J. G. Ammonia assimilation and synthesis of alanine, aspartate, and glutamate in Methanosarcina barkeri and Methanobacterium thermoautotrophicum. J Bacteriol. 1982 Jun;150(3):1357–1365. doi: 10.1128/jb.150.3.1357-1365.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kulaev I. S., Vagabov V. M. Polyphosphate metabolism in micro-organisms. Adv Microb Physiol. 1983;24:83–171. doi: 10.1016/s0065-2911(08)60385-9. [DOI] [PubMed] [Google Scholar]
- Roberton A. M., Wolfe R. S. Adenosine triphosphate pools in Methanobacterium. J Bacteriol. 1970 Apr;102(1):43–51. doi: 10.1128/jb.102.1.43-51.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sauer F. D., Erfle J. D., Mahadevan S. Evidence for an internal electrochemical proton gradient in Methanobacterium thermoautotrophicum. J Biol Chem. 1981 Oct 10;256(19):9843–9848. [PubMed] [Google Scholar]
- Seely R. J., Fahrney D. E. A novel diphospho-P,P'-diester from Methanobacterium thermoautotrophicum. J Biol Chem. 1983 Sep 25;258(18):10835–10838. [PubMed] [Google Scholar]
- Seely R. J., Krueger R. D., Fahrney D. E. Cyclic-2,3-diphosphoglycerate levels in Methanobacterium thermoautotrophicum reflect inorganic phosphate availability. Biochem Biophys Res Commun. 1983 Nov 15;116(3):1125–1128. doi: 10.1016/s0006-291x(83)80259-9. [DOI] [PubMed] [Google Scholar]
- Taylor G. T., Pirt S. J. Nutrition and factors limiting the growth of a methanogenic bacterium (Methanobacterium thermoautotrophicum). Arch Microbiol. 1977 May 13;113(1-2):17–22. doi: 10.1007/BF00428574. [DOI] [PubMed] [Google Scholar]
- Zeikus J. G., Bowen V. G. Comparative ultrastructure of methanogenic bacteria. Can J Microbiol. 1975 Feb;21(2):121–129. doi: 10.1139/m75-019. [DOI] [PubMed] [Google Scholar]
- Zeikus J. G., Bowen V. G. Fine structure of Methanospirillum hungatii. J Bacteriol. 1975 Jan;121(1):373–380. doi: 10.1128/jb.121.1.373-380.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zeikus J. G., Wolfe R. S. Methanobacterium thermoautotrophicus sp. n., an anaerobic, autotrophic, extreme thermophile. J Bacteriol. 1972 Feb;109(2):707–715. doi: 10.1128/jb.109.2.707-713.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]