Abstract
A futile xylitol cycle appears to be responsible for xylitol-mediated inhibition of growth of Lactobacillus casei Cl-16 at the expense of ribitol. The gratuitously induced xylitol-specific phosphoenolpyruvate-dependent phosphotransferase accumulates the pentitol as xylitol-5-phosphate, a phosphatase cleaves the latter, and an export system expels the xylitol. Operation of the cycle rapidly dissipates the ribitol-5-phosphate pool (and ultimately the energy supply of the cell), thereby producing bacteriostasis.
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Selected References
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- Chassy B. M., Thompson J. Regulation and characterization of the galactose-phosphoenolpyruvate-dependent phosphotransferase system in Lactobacillus casei. J Bacteriol. 1983 Jun;154(3):1204–1214. doi: 10.1128/jb.154.3.1204-1214.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chassy B. M., Thompson J. Regulation of lactose-phosphoenolpyruvate-dependent phosphotransferase system and beta-D-phosphogalactoside galactohydrolase activities in Lactobacillus casei. J Bacteriol. 1983 Jun;154(3):1195–1203. doi: 10.1128/jb.154.3.1195-1203.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horder M. Colorimetric determination of orthophosphate in the assay of inorganic pyrophosphatase activity. Anal Biochem. 1972 Sep;49(1):37–47. doi: 10.1016/0003-2697(72)90240-0. [DOI] [PubMed] [Google Scholar]
- London J., Chace N. M. Pentitol metabolism in Lactobacillus casei. J Bacteriol. 1979 Dec;140(3):949–954. doi: 10.1128/jb.140.3.949-954.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- London J., Hausman S. Z. Purification and characterization of the IIIXtl phospho-carrier protein of the phosphoenolpyruvate-dependent xylitol:phosphotransferase found in Lactobacillus casei C183. J Bacteriol. 1983 Nov;156(2):611–619. doi: 10.1128/jb.156.2.611-619.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- London J., Hausman S. Xylitol-mediated transient inhibition of ribitol utilization by Lactobacillus casei. J Bacteriol. 1982 May;150(2):657–661. doi: 10.1128/jb.150.2.657-661.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- London J., Kline K. Aldolase of lactic acid bacteria: a case history in the use of an enzyme as an evolutionary marker. Bacteriol Rev. 1973 Dec;37(4):453–478. doi: 10.1128/br.37.4.453-478.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MIZUSHIMA S., MACHIDA Y., KITAHARA K. QUANTITATIVE STUDIES ON GLYCOLYTIC ENZYMES IN LACTOBACILLUS PLANTARUM. I. CONCENTRATION OF INORGANIC IONS AND COENZYMES IN FERMENTING CELLS. J Bacteriol. 1963 Dec;86:1295–1300. doi: 10.1128/jb.86.6.1295-1300.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reizer J., Novotny M. J., Panos C., Saier M. H., Jr Mechanism of inducer expulsion in Streptococcus pyogenes: a two-step process activated by ATP. J Bacteriol. 1983 Oct;156(1):354–361. doi: 10.1128/jb.156.1.354-361.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reizer J., Panos C. Regulation of beta-galactoside phosphate accumulation in Streptococcus pyogenes by an expulsion mechanism. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5497–5501. doi: 10.1073/pnas.77.9.5497. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reizer J., Saier M. H., Jr Involvement of lactose enzyme II of the phosphotransferase system in rapid expulsion of free galactosides from Streptococcus pyogenes. J Bacteriol. 1983 Oct;156(1):236–242. doi: 10.1128/jb.156.1.236-242.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thompson J., Chassy B. M. Intracellular hexose-6-phosphate:phosphohydrolase from Streptococcus lactis: purification, properties, and function. J Bacteriol. 1983 Oct;156(1):70–80. doi: 10.1128/jb.156.1.70-80.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thompson J., Chassy B. M. Novel phosphoenolpyruvate-dependent futile cycle in Streptococcus lactis: 2-deoxy-D-glucose uncouples energy production from growth. J Bacteriol. 1982 Sep;151(3):1454–1465. doi: 10.1128/jb.151.3.1454-1465.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thompson J. In vivo regulation of glycolysis and characterization of sugar: phosphotransferase systems in Streptococcus lactis. J Bacteriol. 1978 Nov;136(2):465–476. doi: 10.1128/jb.136.2.465-476.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thompson J., Saier M. H., Jr Regulation of methyl-beta-d-thiogalactopyranoside-6-phosphate accumulation in Streptococcus lactis by exclusion and expulsion mechanisms. J Bacteriol. 1981 Jun;146(3):885–894. doi: 10.1128/jb.146.3.885-894.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thompson J., Thomas T. D. Phosphoenolpyruvate and 2-phosphoglycerate: endogenous energy source(s) for sugar accumulation by starved cells of Streptococcus lactis. J Bacteriol. 1977 May;130(2):583–595. doi: 10.1128/jb.130.2.583-595.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thompson J., Torchia D. A. Use of 31P nuclear magnetic resonance spectroscopy and 14C fluorography in studies of glycolysis and regulation of pyruvate kinase in Streptococcus lactis. J Bacteriol. 1984 Jun;158(3):791–800. doi: 10.1128/jb.158.3.791-800.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]