Abstract
Reproducible conditions have been described for quantitative tissue culturing in which all constituents including the tissue itself are added in known quantities to the culture. The conditions for transfer, cutting, and manipulation of tissue are also described. The data show that such culturing can be performed without loss or significant alteration of the nitrogen and phosphorus content of the tissue.
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Selected References
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- Davidson J. N., Waymouth C. Factors influencing the nucleoprotein content of fibroblasts growing in vitro. Biochem J. 1943 Jul;37(2):271–277. doi: 10.1042/bj0370271. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davidson J. N., Waymouth C. The nucleoprotein content of fibroblasts growing in vitro: 2. The effect of tissue extracts. Biochem J. 1945;39(2):188–199. doi: 10.1042/bj0390188. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rigler N. E., Greathouse G. A. AN AUTOMATIC ZERO PIPETTE FOR DISPENSING STERILE CULTURE MEDIA. Science. 1940 Oct 18;92(2390):363–364. doi: 10.1126/science.92.2390.363. [DOI] [PubMed] [Google Scholar]
- Shaw D. T., Kingsland L. C., Brues A. M. A ROLLER BOTTLE TISSUE CULTURE SYSTEM. Science. 1940 Feb 9;91(2354):148–149. doi: 10.1126/science.91.2354.148. [DOI] [PubMed] [Google Scholar]