Abstract
The exchange of potassium between cells and plasma of heparinized human blood has been studied in vitro using the radioactive isotope K42. The changes in cell and plasma specific activity are characteristic of a simple two-compartment system. The mean of seven determinations of the exchange rate at 38°C. is 1.8 per cent of the cellular potassium per hour. The results indicate that at 38°C. the rate is relatively insensitive to oxygenation or reduction of the hemoglobin, and to 1200 r of gamma radiation. With varying temperature the rate follows pseudo first order kinetics with a Q 10 of 2.35. Below 15°C. the rate of loss of potassium exceeds the rate of uptake.
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Selected References
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- HEILBRUNN L. V., WILSON W. L. The effect of heparin on cell division. Proc Soc Exp Biol Med. 1949 Jan;70(1):179–182. doi: 10.3181/00379727-70-16866. [DOI] [PubMed] [Google Scholar]
- Maizels M. Cation control in human erythrocytes. J Physiol. 1949 May 15;108(3):247–263. [PMC free article] [PubMed] [Google Scholar]
- Parpart A. K., Ballentine R. HEMATOCRIT DETERMINATION OF RELATIVE CELL VOLUME. Science. 1943 Dec 17;98(2555):545–545. doi: 10.1126/science.98.2555.545. [DOI] [PubMed] [Google Scholar]
- Parpart A. K., Gregg J. R., Lorenz P. B., Parpart E. R., Chase A. M. WHOLE BLOOD PRESERVATION; A PROBLEM IN GENERAL PHYSIOLOGY. AN IN VITRO ANALYSIS OF THE PROBLEM OF BLOOD STORAGE. J Clin Invest. 1947 Jul;26(4):641–654. doi: 10.1172/JCI101848. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rapoport S., Wing M. DIMENSIONAL, OSMOTIC, AND CHEMICAL CHANGES OF ERYTHROCYTES IN STORED BLOOD. I. BLOOD PRESERVED IN SODIUM CITRATE, NEUTRAL, AND ACID CITRATE-GLUCOSE (ACD) MIXTURES. J Clin Invest. 1947 Jul;26(4):591–615. doi: 10.1172/JCI101843. [DOI] [PMC free article] [PubMed] [Google Scholar]