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. 1950 Nov 20;34(2):225–229. doi: 10.1085/jgp.34.2.225

PHAGE FORMATION IN STAPHYLOCOCCUS MUSCAE CULTURES

VII. PARTIAL PURIFICATION OF THE PROTEIN FACTOR NECESSARY FOR VIRUS SYNTHESIS

Winston H Price 1
PMCID: PMC2147253  PMID: 14824493

Abstract

1. A substance is present in autolyzed pepsin solutions which stimulates the release of phage by some strains of S. muscae when added to Fildes' synthetic medium. 2. The substance is assayed by determining the quantity necessary to increase the phage yield to one-half the maximum value, using the one-step growth curve technique. 3. The substance has been concentrated and partially purified (500-fold) by heavy metal precipitation, butyl alcohol extraction, and absorption on norit. 4. No known amino acid or accessory growth substance tested could replace this substance.

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  1. PRICE W. H. Phage formation in Staphylococcus muscae cultures. VIII. Effect of the protein factor and aspartic acid on virus synthesis with various bacterial strains. J Gen Physiol. 1950 Nov;34(2):231–250. doi: 10.1085/jgp.34.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]

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