Abstract
1. Dialysis of a hemoglobin solution against slightly alkaline buffer (pH 8.68) causes a decrease in heme-heme interaction. The value of n in Hill's equation drops from 2.9 to 2.0, while the oxygen affinity increases slightly. Addition of glutathione largely reverses the effects of dialysis (n rises from 2.0 to 2.5 to 2.6). 2. Addition of the sulfhydryl reagent, p-chloromercuribenzoate (1.8 x 10–3 M), results in a large though not complete blocking of heme-heme interaction. The value of n drops from 2.9 to 1.4. This effect is largely reversed with glutathione (n rises to 2.6).
Full Text
The Full Text of this article is available as a PDF (767.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HUGGINS C., TAPLEY D. F., JENSEN E. V. Sulphydryl-disulphide relationships in the induction of gels in proteins by urea. Nature. 1951 Apr 14;167(4250):592–593. doi: 10.1038/167592a0. [DOI] [PubMed] [Google Scholar]
- INGBAR S. H., KASS E. H. Sulfhydryl content of normal hemoglobin and hemoglobin in sickle cell anemia. Proc Soc Exp Biol Med. 1951 May;77(1):74–76. doi: 10.3181/00379727-77-18683. [DOI] [PubMed] [Google Scholar]
- PAULING L., ITANO H. A. Sickle cell anemia a molecular disease. Science. 1949 Nov 25;110(2865):543–548. doi: 10.1126/science.110.2865.543. [DOI] [PubMed] [Google Scholar]
- RIGGS A. The metamorphosis of hemoglobin in the bullfrog. J Gen Physiol. 1951 Sep;35(1):23–40. doi: 10.1085/jgp.35.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]