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. 1952 Nov 20;36(2):227–241. doi: 10.1085/jgp.36.2.227

SOME ASPECTS OF THE DESOXYRIBONUCLEASE ACTIVITIES OF ANIMAL TISSUES

V Allfrey 1, A E Mirsky 1
PMCID: PMC2147360  PMID: 13011279

Abstract

It has been found that many animal tissues contain "acid" desoxyribonucleases with pH optima near 5.2. A chemical method for the determination of this activity is described. The pancreatic desoxyribonuclease crystallized by Kunitz and shown to have a neutral pH optimum occurs in the pancreas together with the "acid" enzyme, but only the "neutral" enzyme occurs in the pancreatic juice. The ratio of "neutral" to "acid" DNAase activities in the pancreas is greater than 200, but in all other tissues examined there is no appreciable concentration of the neutral enzyme. It is concluded that neutral DNAase, like trypsin or lipase, has a digestive function. Some problems in the activation of the secretory enzyme in neutral pancreatic extracts are described. This activation can be interpreted in terms of a specific inhibitor or an inactive form of the enzyme. A comparison of the "acid" DNAase activities of different organs of the calf, horse, chicken, mouse, and rat indicates a possible connection between the DNAase concentration of a tissue and its capacity for proliferation or regeneration. However, the comparative DNAase activities of fetal and adult tissues do not support the view that DNAase function is limited to some simple role in the mechanics of cell division. Studies on the incorporation of glycine-N15 into the desoxypentose nucleic acids of avian red cells, and mouse liver, pancreas, and kidney show that the N15 uptake into the DNA of the chromosome is most rapid in tissues with high DNAase concentrations. No N15 incorporation is observed in the DNA of avian red cells, which have negligible concentrations of the enzyme. The analyses of tissues and nuclei isolated in non-aqueous media show that the bulk of the enzyme occurs in the cytoplasm of the cell, and that nuclear concentrations vary from tissue to tissue. A theory relating the DNAase activity of the cell to its over-all desoxypentose nucleotide metabolism is discussed. No evidence has been found for the presence of inhibitors of the "acid" DNAase in animal tissues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLFREY V., MIRSKY A. E. The incorporation of N15 glycine by avian erythrocytes and reticulocytes in vitro. J Gen Physiol. 1952 Jul;35(6):841–846. doi: 10.1085/jgp.35.6.841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ALLFREY V., STERN H., MIRSKY A. E., SAETREN H. The isolation of cell nuclei in non-aqueous media. J Gen Physiol. 1952 Jan;35(3):529–554. doi: 10.1085/jgp.35.3.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BROWN K. D., JACOBS G., LASKOWSKI M. The distribution of nucleode polymerases it calf thymus fractions. J Biol Chem. 1952 Jan;194(1):445–454. [PubMed] [Google Scholar]
  4. DALY M. M., ALLFREY V. G., MIRSKY A. E. Uptake of glycine-N15 by components of cell nuclei. J Gen Physiol. 1952 Nov;36(2):173–179. doi: 10.1085/jgp.36.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HENSTELL H. H., FREEDMAN R. I., GINSBURG B. An inhibitor of desoxyribonuclease in human white blood and bone marrow cells, and its relationship to cellular maturity. Cancer Res. 1952 May;12(5):346–349. [PubMed] [Google Scholar]
  6. HOFF-JORGENSEN E., ZEUTHEN E. Evidence of cytoplasmic deoxyribosides in the frog's egg. Nature. 1952 Feb 9;169(4293):245–246. doi: 10.1038/169245a0. [DOI] [PubMed] [Google Scholar]
  7. Henstell H. H., Freedman R. I. An Inhibitor of Desoxyribonuclease in Human White Blood Cells and Bone Marrow Cells and its Relationship to Cellular Maturity. Science. 1952 Mar 28;115(2987):357–358. doi: 10.1126/science.115.2987.357. [DOI] [PubMed] [Google Scholar]
  8. KUNITZ M. Crystalline desoxyribonuclease; digestion of thymus nucleic acid; the kinetics of the reaction. J Gen Physiol. 1950 Mar;33(4):363–377. doi: 10.1085/jgp.33.4.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KUNITZ M. Crystalline desoxyribonuclease; isolation and general properties; spectrophotometric method for the measurement of desoxyribonuclease activity. J Gen Physiol. 1950 Mar;33(4):349–362. doi: 10.1085/jgp.33.4.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KURNICK N. B. The determination of desoxyribonuclease activity by methyl green; application to serum. Arch Biochem. 1950 Nov;29(1):41–53. [PubMed] [Google Scholar]
  11. MAVER M. E., GRECO A. E. The nuclease activities of cathepsin preparations from calf spleen and thymus. J Biol Chem. 1949 Dec;181(2):861–870. [PubMed] [Google Scholar]
  12. MAZIA D. The distribution of desoxyribonuclease in the developing embryo, Arbacia punctulata. J Cell Physiol. 1949 Aug;34(1):17–32. doi: 10.1002/jcp.1030340103. [DOI] [PubMed] [Google Scholar]
  13. MIRSKY A. E., RIS H. Variable and constant components of chromosomes. Nature. 1949 Apr 30;163(4148):666–666. doi: 10.1038/163666a0. [DOI] [PubMed] [Google Scholar]
  14. Martland M., Robison R. Possible Significance of Hexosephosphoric Esters in Ossification: Part VI. Phosphoric Esters in Blood-Plasma. Biochem J. 1926;20(4):847–855. doi: 10.1042/bj0200847. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WEBB M. Use of deoxyribonuclease inhibitors in the isolation of deoxyribonucleic acids. Nature. 1952 Mar 8;169(4297):417–417. doi: 10.1038/169417a0. [DOI] [PubMed] [Google Scholar]

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