Abstract
1. The uptake of glycine-N15 by components of cell nuclei was studied. The nuclear components were derived both from tissues with high metabolic rates-mammalian liver, kidney, and pancreas-and from cells with relatively low rates of metabolism-avian erythrocytes and echinoderm sperm. N15 uptake by nuclear components of liver, kidney, and pancreas was far more rapid than by those of erythrocytes and sperm. 2. The nuclear components of liver, kidney, and pancreas for which measurements were made were DNA, histone, and residual protein of chromatin. Uptake into DNA was low, into histone higher, and into residual protein much higher still, being comparable with that into mixed cytoplasmic protein. 3. A comparison of the uptake of N15 by the chromosomal components, histone and DNA of liver, pancreas, and kidney showed that chromosomal "activity" varies in different cells and also in the same cell depending upon its over-all activity.
Full Text
The Full Text of this article is available as a PDF (484.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALLFREY V., MIRSKY A. E. The incorporation of N15 glycine by avian erythrocytes and reticulocytes in vitro. J Gen Physiol. 1952 Jul;35(6):841–846. doi: 10.1085/jgp.35.6.841. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ALLFREY V., STERN H., MIRSKY A. E., SAETREN H. The isolation of cell nuclei in non-aqueous media. J Gen Physiol. 1952 Jan;35(3):529–554. doi: 10.1085/jgp.35.3.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DALY M. M., MIRSKY A. E., RIS H. The amino acid composition and some properties of histones. J Gen Physiol. 1951 Mar 20;34(4):439–450. doi: 10.1085/jgp.34.4.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FURST S. S., BROWN G. B. On the role of glycine and adenine as precursors of nucleic acid purines. J Biol Chem. 1951 Jul;191(1):239–247. [PubMed] [Google Scholar]
- MIRSKY A. E., RIS H. The composition and structure of isolated chromosomes. J Gen Physiol. 1951 May;34(5):475–492. doi: 10.1085/jgp.34.5.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MIRSKY A. E., RIS H. Variable and constant components of chromosomes. Nature. 1949 Apr 30;163(4148):666–666. doi: 10.1038/163666a0. [DOI] [PubMed] [Google Scholar]
- REICHARD P. On the nitrogen turnover in purines from polynucleotides determined with glycine N15. J Biol Chem. 1949 Jun;179(2):773–781. [PubMed] [Google Scholar]
- TYLER A., ATKINSON E. Prolongation of the fertilizing capacity of seaurchin spermatozoa by amino acids. Science. 1950 Dec 29;112(2922):783–785. doi: 10.1126/science.112.2922.783. [DOI] [PubMed] [Google Scholar]