Abstract
1. A procedure for isolating nuclei of the wheat germ in non-aqueous media has been described. 2. Such nuclei were shown to constitute about 50 per cent of the protoplasmic mass and to have a ribonucleic acid content of an order equivalent to that of the cytoplasm. 3. Studies of the distribution of the enzymes—aldolase, phosphoglyceraldehyde dehydrogenase, enolase, and pyruvate kinase—have revealed that the nuclei are the most vigorous sites of glycolytic activity. 4. Analysis of the DPN content of the nuclei in calf tissues—liver, pancreas, and heart—pointed to the probability that glycolytic activity is a characteristic common to many nuclei. 5. The significance of glycolytic activity to nuclear function has been discussed and some suggestive comparisons made between the two energy-yielding systems of glycolytic and oxidative phosphorylation.
Full Text
The Full Text of this article is available as a PDF (1.1 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALLFREY V., STERN H., MIRSKY A. E., SAETREN H. The isolation of cell nuclei in non-aqueous media. J Gen Physiol. 1952 Jan;35(3):529–554. doi: 10.1085/jgp.35.3.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LANG K., SIEBERT G. Untersuchungen über Stoffwechselvorgänge in isolierten Zellkernen. 5. Uber Energielieferung durch Glykolyse und ATP-Spaltung sowie über das Vorkommen von Dehydrasen in isolierten Zellkernen. Biochem Z. 1951 Dec;322(3):196–204. [PubMed] [Google Scholar]
- RACKER E. Enzymatic synthesis of deoxypentose phosphate. Nature. 1951 Mar 10;167(4245):408–409. doi: 10.1038/167408b0. [DOI] [PubMed] [Google Scholar]
- STERN H., ALLFREY V., MIRSKY A. E., SAETREN H. Some enzymes of isolated nuclei. J Gen Physiol. 1952 Jan;35(3):559–578. doi: 10.1085/jgp.35.3.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
- UTTER M. F., WOOD H. G. Mechanisms of fixation of carbon dioxide by heterotrophes and autotrophs. Adv Enzymol Relat Subj Biochem. 1951;12:41–151. doi: 10.1002/9780470122570.ch2. [DOI] [PubMed] [Google Scholar]