Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Oct;160(1):466–469. doi: 10.1128/jb.160.1.466-469.1984

Effects of cultivation gas phase on hydrogenase of the acetogen Clostridium thermoaceticum.

R Kellum, H L Drake
PMCID: PMC214747  PMID: 6434525

Abstract

The effect of cultivation gas phase on the expression and activity of hydrogenase in heterotrophic cultures of Clostridium thermoaceticum was examined. Of the five gas phases tested, hydrogenase was maximal from cells cultivated under CO. Correlations were observed between the level of hydrogenase and the evolution of H2 by growing cultures. Activity stains of polyacrylamide gels revealed a single hydrogenase band in CO2 cells and multiple hydrogenase bands in CO cells.

Full text

PDF
466

Images in this article

468Image
on p.468

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Diekert G. B., Thauer R. K. Carbon monoxide oxidation by Clostridium thermoaceticum and Clostridium formicoaceticum. J Bacteriol. 1978 Nov;136(2):597–606. doi: 10.1128/jb.136.2.597-606.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Drake H. L. Demonstration of hydrogenase in extracts of the homoacetate-fermenting bacterium Clostridium thermoaceticum. J Bacteriol. 1982 May;150(2):702–709. doi: 10.1128/jb.150.2.702-709.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Drake H. L., Hu S. I., Wood H. G. Purification of five components from Clostridium thermoaceticum which catalyze synthesis of acetate from pyruvate and methyltetrahydrofolate. Properties of phosphotransacetylase. J Biol Chem. 1981 Nov 10;256(21):11137–11144. [PubMed] [Google Scholar]
  5. Drake H. L. Occurrence of nickel in carbon monoxide dehydrogenase from Clostridium pasteurianum and Clostridium thermoaceticum. J Bacteriol. 1982 Feb;149(2):561–566. doi: 10.1128/jb.149.2.561-566.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fontaine F. E., Peterson W. H., McCoy E., Johnson M. J., Ritter G. J. A New Type of Glucose Fermentation by Clostridium thermoaceticum. J Bacteriol. 1942 Jun;43(6):701–715. doi: 10.1128/jb.43.6.701-715.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Friedrich C. G. Depression of hydrogenase during limitation of electron donors and derepression of ribulosebisphosphate carboxylase during carbon limitation of Alcaligenes eutrophus. J Bacteriol. 1982 Jan;149(1):203–210. doi: 10.1128/jb.149.1.203-210.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hu S. I., Drake H. L., Wood H. G. Synthesis of acetyl coenzyme A from carbon monoxide, methyltetrahydrofolate, and coenzyme A by enzymes from Clostridium thermoaceticum. J Bacteriol. 1982 Feb;149(2):440–448. doi: 10.1128/jb.149.2.440-448.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jacobson F. S., Daniels L., Fox J. A., Walsh C. T., Orme-Johnson W. H. Purification and properties of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum. J Biol Chem. 1982 Apr 10;257(7):3385–3388. [PubMed] [Google Scholar]
  10. Krasna A. I. Regulation of hydrogenase activity in enterobacteria. J Bacteriol. 1980 Dec;144(3):1094–1097. doi: 10.1128/jb.144.3.1094-1097.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lundie L. L., Jr, Drake H. L. Development of a minimally defined medium for the acetogen Clostridium thermoaceticum. J Bacteriol. 1984 Aug;159(2):700–703. doi: 10.1128/jb.159.2.700-703.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Merberg D., O'Hara E. B., Maier R. J. Regulation of hydrogenase in Rhizobium japonicum: analysis of mutants altered in regulation by carbon substrates and oxygen. J Bacteriol. 1983 Dec;156(3):1236–1242. doi: 10.1128/jb.156.3.1236-1242.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pecher A., Zinoni F., Jatisatienr C., Wirth R., Hennecke H., Böck A. On the redox control of synthesis of anaerobically induced enzymes in enterobacteriaceae. Arch Microbiol. 1983 Nov;136(2):131–136. doi: 10.1007/BF00404787. [DOI] [PubMed] [Google Scholar]
  14. Pezacka E., Wood H. G. The synthesis of acetyl-CoA by Clostridium thermoaceticum from carbon dioxide, hydrogen, coenzyme A and methyltetrahydrofolate. Arch Microbiol. 1984 Jan;137(1):63–69. doi: 10.1007/BF00425809. [DOI] [PubMed] [Google Scholar]
  15. Ragsdale S. W., Clark J. E., Ljungdahl L. G., Lundie L. L., Drake H. L. Properties of purified carbon monoxide dehydrogenase from Clostridium thermoaceticum, a nickel, iron-sulfur protein. J Biol Chem. 1983 Feb 25;258(4):2364–2369. [PubMed] [Google Scholar]
  16. Schaupp A., Ljungdahl L. G. Purification and properties of acetate kinase from Clostridium thermoaceticum. Arch Microbiol. 1974;100(2):121–129. doi: 10.1007/BF00446312. [DOI] [PubMed] [Google Scholar]
  17. Spiller H., Bookjans G., Shanmugam K. T. Regulation of hydrogenase activity in vegetative cells of Anabaena variabilis. J Bacteriol. 1983 Jul;155(1):129–137. doi: 10.1128/jb.155.1.129-137.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yamamoto I., Saiki T., Liu S. M., Ljungdahl L. G. Purification and properties of NADP-dependent formate dehydrogenase from Clostridium thermoaceticum, a tungsten-selenium-iron protein. J Biol Chem. 1983 Feb 10;258(3):1826–1832. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES