Abstract
1. A procedure has been developed for the preparation of an active concentrate from the myxomycete, Physarum polycephalum. This concentrate responds with a lowered viscosity to the addition of small amounts of ATP. The preparation recovers in viscosity, and the process may be repeated. 2. In the most active concentrates, 75 per cent of the non-dialyzable material moves as a single boundary both in the descending limb in electrophoresis and in the ultracentrifuge. It contains about 10 per cent ribonucleic acid, which is at least in part reversibly bound to the protein. 3. The active material has been designated myxomyosin because of its origin and its similarity to actomyosin in ATP response.
Full Text
The Full Text of this article is available as a PDF (630.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- GORNALL A. G., BARDAWILL C. J., DAVID M. M. Determination of serum proteins by means of the biuret reaction. J Biol Chem. 1949 Feb;177(2):751–766. [PubMed] [Google Scholar]
- LOEWY A. An actomyosin-like substance from the plasmodium of a myxomycete. J Cell Physiol. 1952 Aug;40(1):127–156. doi: 10.1002/jcp.1030400109. [DOI] [PubMed] [Google Scholar]
- TS'O P. O., BONNER J., EGGMAN L., VINOGRAD J. Observations on an ATP-sensitive protein system from the plasmodia of a myxomycete. J Gen Physiol. 1956 Jan 20;39(3):325–347. doi: 10.1085/jgp.39.3.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WYATT G. R. Recognition and estimation of 5-methylcytosine in nucleic acids. Biochem J. 1951 May;48(5):581–584. doi: 10.1042/bj0480581. [DOI] [PMC free article] [PubMed] [Google Scholar]