Abstract
1. Oxidative dissimilation has been studied in enzymes from the honey bee. Using mitochondria isolated from the thoraces, complete oxidation of most of the TCA cycle members has been shown. 2. The presence of the acetate-activating enzyme, citrate-condensing enzyme, isocitric dehydrogenase, α-ketoglutarate dehydrogenase, glucose-6-phosphate, and 6-phosphogluconic dehydrogenase has been demonstrated and the cofactor requirements established. 3. The oxidation of isocitric acid has been shown to be either non-specific for the D- or L-isomer, or the presence of a racemase is indicated. 4. The presence of the pentose cycle is indicated in the soluble portion of the thoracic homogenate.
Full Text
The Full Text of this article is available as a PDF (536.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brummond D. O., Burris R. H. Transfer of C By Lupine Mitochondria Through Reactions of the Tricarboxylic Acid Cycle. Proc Natl Acad Sci U S A. 1953 Aug;39(8):754–759. doi: 10.1073/pnas.39.8.754. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHEFURKA W. The occurrence of a direct oxidative pathway of carbohydrate metabolism in the fly Musca domestica L. Biochim Biophys Acta. 1955 Jun;17(2):294–296. doi: 10.1016/0006-3002(55)90375-9. [DOI] [PubMed] [Google Scholar]
- KERBS H. A., GURIN S., EGGLESTON L. V. The pathway of oxidation of acetate in baker's yeast. Biochem J. 1952 Aug;51(5):614–628. doi: 10.1042/bj0510614. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PARDEE A. B., POTTER V. R. Malonate inhibition of oxidations in the Krebs tricarboxylic acid cycle. J Biol Chem. 1949 Mar;178(1):241–250. [PubMed] [Google Scholar]
- SACKTOR B. Investigations on the mitochondria of the house fly, Musca domestica L. I. Adenosinetriphosphatases. J Gen Physiol. 1953 Jan;36(3):371–387. doi: 10.1085/jgp.36.3.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SEEGMILLER J. E., HORECKER B. L. The synthesis of glucose-6-phosphate and 6-phosphogluconate. J Biol Chem. 1951 Sep;192(1):175–180. [PubMed] [Google Scholar]
- WATANABE M. I., WILLIAMS C. M. Mitochondria in the flight muscles of insects. II. Effects of the medium on the size form, and organization of isolated sarcosomes. J Gen Physiol. 1953 Sep;37(1):71–90. doi: 10.1085/jgp.37.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]