Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Nov;160(2):656–661. doi: 10.1128/jb.160.2.656-661.1984

Regulation of amino acid utilization in Neurospora crassa: effect of nmr-1 and ms-5 mutations.

R M DeBusk, S Ogilvie
PMCID: PMC214785  PMID: 6238946

Abstract

The effect of the nmr-1 and ms-5 mutations, which lead to insensitivity to glutamine-mediated nitrogen metabolite repression, was examined with respect to extracellular deaminase production by Neurospora crassa. Deaminase production normally requires nitrogen limitation, but these mutations eliminated this requirement and allowed production of deaminase activity under nitrogen metabolite repressing conditions. Demonstration of normal glutamine transport by both strains eliminated the possibility that these mutations exerted their effects through repressor exclusion. We have proposed a new working model for nitrogen regulation in Neurospora based on the findings that these mutations affected a nitrogen-regulated activity in addition to those activities originally reported and that the mutations are genetically very closely linked and likely allelic.

Full text

PDF
656

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arst H. N., Jr, Cove D. J. Nitrogen metabolite repression in Aspergillus nidulans. Mol Gen Genet. 1973 Nov 2;126(2):111–141. doi: 10.1007/BF00330988. [DOI] [PubMed] [Google Scholar]
  2. Arst H. N., Jr, Scazzocchio C. Initiator constitutive mutation with an 'up-promoter' effect in Aspergillus nidulans. Nature. 1975 Mar 6;254(5495):31–34. doi: 10.1038/254031a0. [DOI] [PubMed] [Google Scholar]
  3. Coddington A. Biochemical studies on the nit mutants of Neurospora crassa. Mol Gen Genet. 1976 May 7;145(2):195–206. doi: 10.1007/BF00269594. [DOI] [PubMed] [Google Scholar]
  4. Cove D. J. Chlorate toxicity in Aspergillus nidulans. Studies of mutants altered in nitrate assimilation. Mol Gen Genet. 1976 Jul 23;146(2):147–159. doi: 10.1007/BF00268083. [DOI] [PubMed] [Google Scholar]
  5. Cove D. J. Genetic studies of nitrate assimilation in Aspergillus nidulans. Biol Rev Camb Philos Soc. 1979 Aug;54(3):291–327. doi: 10.1111/j.1469-185x.1979.tb01014.x. [DOI] [PubMed] [Google Scholar]
  6. DeBusk R. M., Brown D. T., DeBusk A. G., Penderghast R. D. Alternate mechanism for amino acid entry into Neurospora crassa: extracellular deamination and subsequent keto acid transport. J Bacteriol. 1981 Apr;146(1):163–169. doi: 10.1128/jb.146.1.163-169.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DeBusk R. M., DeBusk A. G. Physiological and regulatory properties of the general amino acid transport system of Neurospora crassa. J Bacteriol. 1980 Jul;143(1):188–197. doi: 10.1128/jb.143.1.188-197.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DeBusk R. M., Ogilvie-Villa S. Physiological adaptation to the loss of amino acid transport ability. J Bacteriol. 1982 Oct;152(1):545–548. doi: 10.1128/jb.152.1.545-548.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DeBusk R. M., Ogilvie S. Nitrogen regulation of amino acid utilization by Neurospora crassa. J Bacteriol. 1984 Nov;160(2):493–498. doi: 10.1128/jb.160.2.493-498.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. DeBusk R. M., Ogilvie S. Participation of an extracellular deaminase in amino acid utilization by Neurospora crassa. J Bacteriol. 1984 Aug;159(2):583–589. doi: 10.1128/jb.159.2.583-589.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dunn-Coleman N. S., Tomsett A. B., Garrett R. H. The regulation of nitrate assimilation in Neurospora crassa: biochemical analysis of the nmr-1 mutants. Mol Gen Genet. 1981;182(2):234–239. doi: 10.1007/BF00269663. [DOI] [PubMed] [Google Scholar]
  12. Facklam T. J., Marzluf G. A. Nitrogen regulation of amino acid catabolism in Neurospora crassa. Biochem Genet. 1978 Apr;16(3-4):343–354. doi: 10.1007/BF00484090. [DOI] [PubMed] [Google Scholar]
  13. Hynes M. J. Studies on the role of the areA gene in the regulation of nitrogen catabolism in Aspergillus nidulans. Aust J Biol Sci. 1975 Jun;28(3):301–313. doi: 10.1071/bi9750301. [DOI] [PubMed] [Google Scholar]
  14. Marzluf G. A. Regulation of nitrogen metabolism and gene expression in fungi. Microbiol Rev. 1981 Sep;45(3):437–461. doi: 10.1128/mr.45.3.437-461.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nell J. H., DeBusk A. G. Amino acid transport during development of Neurospora crassa conidia: substrate repression. Biochem Genet. 1974 Mar;11(3):183–203. doi: 10.1007/BF00486055. [DOI] [PubMed] [Google Scholar]
  16. Ogilvie-Villa S., DeBusk R. M., DeBusk A. G. Characterization of 2-aminoisobutyric acid transport in Neurospora crassa: a general amino acid permease-specific substrate. J Bacteriol. 1981 Sep;147(3):944–948. doi: 10.1128/jb.147.3.944-948.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Premakumar R., Sorger G. J., Gooden D. Physiological characterization of a Neurospora crassa mutant with impaired regulation of nitrate reductase. J Bacteriol. 1980 Nov;144(2):542–551. doi: 10.1128/jb.144.2.542-551.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rand K. N., Arst H. N., Jr A mutation in Aspergillus nidulans which affects the regulation of nitrite reductase and is tightly linked to its structural gene. Mol Gen Genet. 1977 Sep 21;155(1):67–75. doi: 10.1007/BF00268562. [DOI] [PubMed] [Google Scholar]
  19. Reinert W. R., Marzluf G. A. Genetic and metabolic control of the purine catabolic enzymes of Neurospora crasse. Mol Gen Genet. 1975 Aug 5;139(1):39–55. doi: 10.1007/BF00267994. [DOI] [PubMed] [Google Scholar]
  20. Tomsett A. B., Dunn-Coleman N. S., Garrett R. H. The regulation of nitrate assimilation in Neurospora crassa: the isolation and genetic analysis of nmr-1 mutants. Mol Gen Genet. 1981;182(2):229–233. doi: 10.1007/BF00269662. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES