Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Nov;160(2):718–723. doi: 10.1128/jb.160.2.718-723.1984

Molecular cloning of a malyl coenzyme A lyase gene from Pseudomonas sp. strain AM1, a facultative methylotroph.

G L Fulton, D N Nunn, M E Lidstrom
PMCID: PMC214796  PMID: 6094488

Abstract

A genomic library containing HindIII partial digests of Pseudomonas sp. strain AM1 DNA was constructed in the broad-host-range cosmid pVK100. PCT57, a Pseudomonas sp. strain AM1 methanol mutant deficient in malyl coenzyme A lyase activity, was complemented to a methanol-positive phenotype by mobilization of the pVK100 library into PCT57 recipients with the ColE1/RK2 mobilizing plasmid pRK2013. Six different complemented isolates all contained a recombinant plasmid carrying the same 19.6-kilobase-pair Pseudomonas sp. strain AM1 DNA insert. Subcloning and complementation analysis demonstrated that the gene deficient in PCT57 (mcl-1) was located in a 1.6-kilobase-pair region within a 7.4-kilobase-pair EcoRI-HindIII fragment.

Full text

PDF
718

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Backman K., Ptashne M., Gilbert W. Construction of plasmids carrying the cI gene of bacteriophage lambda. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4174–4178. doi: 10.1073/pnas.73.11.4174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blackmore M. A., Quayle J. R. Microbial growth on oxalate by a route not involving glyoxylate carboligase. Biochem J. 1970 Jun;118(1):53–59. doi: 10.1042/bj1180053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dunstan P. M., Anthony C., Drabble W. T. Microbial metabolism of C 1 and C 2 compounds. The role of glyoxylate, glycollate and acetate in the growth of Pseudomonas AM1 on ethanol and on C 1 compounds. Biochem J. 1972 Jun;128(1):107–115. doi: 10.1042/bj1280107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Figurski D. H., Helinski D. R. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648–1652. doi: 10.1073/pnas.76.4.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hacking A. J., Quayle J. R. Purification and properties of malyl-coenzyme A lyase from Pseudomonas AM1. Biochem J. 1974 May;139(2):399–405. doi: 10.1042/bj1390399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holloway B. W. Plasmids that mobilize bacterial chromosome. Plasmid. 1979 Jan;2(1):1–19. doi: 10.1016/0147-619x(79)90002-7. [DOI] [PubMed] [Google Scholar]
  8. Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
  9. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnson P. A., Quayle J. R. Microbial growth on C-1 compounds. 6. Oxidation of methanol, formaldehyde and formate by methanol-grown Pseudomonas AM-1. Biochem J. 1964 Nov;93(2):281–290. doi: 10.1042/bj0930281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Knauf V. C., Nester E. W. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti plasmid. Plasmid. 1982 Jul;8(1):45–54. doi: 10.1016/0147-619x(82)90040-3. [DOI] [PubMed] [Google Scholar]
  12. Large P. J., Quayle J. R. Microbial growth on C(1) compounds. 5. Enzyme activities in extracts of Pseudomonas AM1. Biochem J. 1963 May;87(2):386–396. doi: 10.1042/bj0870386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McNerney T., O'connor M. L. Regulation of enzymes associated with C-1 metabolism in three facultative methylotrophs. Appl Environ Microbiol. 1980 Aug;40(2):370–375. doi: 10.1128/aem.40.2.370-375.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. O'Connor M. L., Hanson R. S. Serine transhydroxymethylase isoenzymes from a facultative methylotroph. J Bacteriol. 1975 Nov;124(2):985–996. doi: 10.1128/jb.124.2.985-996.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PEEL D., QUAYLE J. R. Microbial growth on C1 compounds. I. Isolation and characterization of Pseudomonas AM 1. Biochem J. 1961 Dec;81:465–469. doi: 10.1042/bj0810465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Salem A. R., Hacking A. J., Quayle J. R. Cleavage of malyl-Coenzyme A into acetyl-Coenzyme A and glyoxylate by Pseudomonas AM1 and other C1-unit-utilizing bacteria. Biochem J. 1973 Sep;136(1):89–96. doi: 10.1042/bj1360089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Salem A. R., Hacking A. J., Quayle J. R. Lack of malyl-CoA lyase in a mutant of Pseudomonas AM1. J Gen Microbiol. 1974 Apr;81(2):525–527. doi: 10.1099/00221287-81-2-525. [DOI] [PubMed] [Google Scholar]
  19. Sancar A., Rupert C. S. Determination of plasmid molecular weights from ultraviolet sensitivities. Nature. 1978 Mar 30;272(5652):471–472. doi: 10.1038/272471a0. [DOI] [PubMed] [Google Scholar]
  20. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  21. Sternberg N., Tiemeier D., Enquist L. In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1. Gene. 1977 May;1(3-4):255–280. doi: 10.1016/0378-1119(77)90049-x. [DOI] [PubMed] [Google Scholar]
  22. Toukdarian A. E., Lidstrom M. E. DNA hybridization analysis of the nif region of two methylotrophs and molecular cloning of nif-specific DNA. J Bacteriol. 1984 Mar;157(3):925–930. doi: 10.1128/jb.157.3.925-930.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Toukdarian A. E., Lidstrom M. E. Molecular construction and characterization of nif mutants of the obligate methanotroph Methylosinus sp. strain 6. J Bacteriol. 1984 Mar;157(3):979–983. doi: 10.1128/jb.157.3.979-983.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Weaver C. A., Redborg A. H., Konisky J. Plasmid-determined immunity of Escherichia coli K-12 to colicin Ia Is mediated by a plasmid-encoded membrane protein. J Bacteriol. 1981 Dec;148(3):817–828. doi: 10.1128/jb.148.3.817-828.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES