Figure 2.—

mrp-4(−) suppresses glo phenotypes. The glo mutant's apt-6(−), glo-4(−), and pgp-2(−) mislocalize birefringent material into the intestinal lumen of pretzel-stage embryos (B, F, and J). glo mutants lacking the activity of mrp-4 typically did not contain birefringent material in the lumen of pretzel-stage embryos (D, H, and L). In A–L the intestinal lumen is marked with a solid arrow and intestinal cells are located between the solid arrowheads. apt-6(−), glo-4(−), and pgp-2(−) embryos elongated to 4-fold in length prior to retracting to 3- to 3.5-fold (M–O). Retraction typically coincided with the appearance of birefringent material in the intestinal lumen (arrow denotes average time of appearance). In M–O the length of individual embryos was monitored every 45 min starting at the 1.5-fold stage, and the bars represent the 95% confidence interval. (P) The survival of apt-6(−), glo-4(−), and pgp-2(−) mutants placed as embryos on 50 mm or 300 mm NaCl plates after 48 hr. The survival of mrp-4(−) double mutants was substantially higher on 300 mm NaCl media than the single glo mutants (*P < .01, t-test). Bars represent the 95% confidence limit.