TABLE 2.
mrp-4(−) and wht-2(−) modify the mislocalization of birefringence exhibited by glo mutants
| Genotype | % of embryos lacking birefringent material in intestinal cells | % of embryos containing birefringent material in the intestinal lumen | n |
|---|---|---|---|
| Wild typea | 0 | 0 | 45 |
| glo-1(zu437)a | 100 | 15 | 97 |
| glo-1(zu437); mrp-4(RNAi) | 100 | 6 | 32 |
| glo-1(zu437); wht-2(RNAi) | 100 | 13 | 134 |
| glo-4(ok623)a | 100 | 53 | 108 |
| glo-4(ok623); mrp-4(RNAi) | 100 | 4 | 26 |
| glo-4(ok623); mrp-4(ok1095)b | 100 | 0 | 200 |
| glo-4(ok623); wht-2(RNAi) | 100 | 53 | 103 |
| pgp-2(kx48)ac | 0 | 0 | 130 |
| pgp-2(kx48); mrp-4(RNAi) | 90 | 0 | 77 |
| pgp-2(kx48); mrp-4(ok1095)b | 98 | 0 | 246 |
| pgp-2(kx48); wht-2(RNAi) | 96 | 57 | 141 |
| pgp-2(kx48); mrp-4(ok1095); wht-2(RNAi)b | 100 | 11 | 35 |
| apt-6(ok429)a | 0 | 16 | 120 |
| apt-6(ok429); mrp-4(RNAi) | 100 | 0 | 25 |
| apt-6(ok429); mrp-4(ok1095)b | 100 | 0 | 107 |
| apt-6(ok429); wht-2(RNAi) | 100 | 58 | 153 |
| apt-6(ok429); mrp-4(ok1095); wht-2(RNAi)b | 100 | 15 | 34 |
| apt-7(tm920)a | 1 | 35 | 97 |
| apt-7(tm920); mrp-4(RNAi) | 66 | 10 | 39 |
| apt-7(tm920); wht-2(RNAi) | 22 | 58 | 71 |
All strains were grown at 22°. Later than twofold-stage embryos were scored for the presence of birefringent material in intestinal cells and in the intestinal lumen using polarization microscopy. n, the number of embryos scored.
Strains were grown on RNAi plates expressing double-stranded RNA against F33E2.4, a gene not required for the formation of birefringent gut granules.
Strains contained a linked dpy-5(e61) or dpy-11(e224) marker that did not affect the presence of localization of birefringent material.
Zero percent and 23% (n = 117) of pgp-2(kx48) embryos derived from adults grown on regular NGM plates lacked birefringent material in intestinal cells and contained birefringent material in the intestinal lumen, respectively.