Figure 3.—

N-terminal processing of the fc125 DEC-1 proprotein. (A) A schematic of fc106, fc125, and their cleaved derivatives (s80, s25, s20, s60, and s95) along with the positions of the open reading frames used to raise the Nfc106 and Ns80 antisera. (B and C) Lanes from Western blots of SDS-soluble proteins from wild-type (wt) or dec-1⁴ mutant egg chambers (fs). (B) Proteins recognized by the Nfc106 antiserum. (C) Lanes shown in A stripped and reincubated with the Ns80 antiserum. (B) The positions of fc125 and its C-terminal derivative s95 and fc106 and its C-terminal derivative s80 are indicated to the left. CR denotes non-DEC-1 related cross-reacting material that is present in DEC-1 protein null mutants (not shown). A transient N-terminal derivative (*) and s25 were recognized by the Nfc106 antiserum. (C) Positions of the C-terminal s95 and s80 derivatives and the N-terminal s20 derivative recognized by the Ns80 antiserum are shown to the right. Egg chamber stages are indicated at the bottom of each lane.