TABLE 1.
Gene-specific primers used in real-time PCR
| Transcript | Primer | Primer sequencea | Product size (bp) (cDNA) |
|---|---|---|---|
| rp49 | rp49 fw | 5′-GTTATGCCAAATTGTCG^CACA | 123 |
| rp49 rev | 5′-GGCGGGTACGTTTGTTGG | ||
| period | per fw | 5′-ACGAAAACACTCTTAAG^CCCAA | 140 |
| per rev | 5′-TTTGCTGTTGTCGTTCTCCTG | ||
| timelessb | tim fw | 5′-TGTTGCTCTTGATACTGGATAGTG | 118 |
| tim rev | 5′-AGCAGCATGCCATAGAAGTG | ||
| cryptochrome | cry fw | 5′-TGGGTTAGTTCATCGGCATTTG | 157 |
| cry rev | 5′-GCCAGGGTTCATGG^ATAAACAG | ||
| vrille | vri fw | 5′-AATGAGGCCACAAATG^TTCAC | 156 |
| vri rev | 5′-GGCGCTGACCTGCTGTTT | ||
| cycle | cyc fw | 5′-CCTGTTCATAGATCAAAG^AGCC | 111 |
| cyc rev | 5′-TCAGCCAAGGCGGGTAT | ||
| Clock | clk fw | 5′-TTATATCACCCCTACCG^CATC | 100 |
| clk rev | 5′-TTACGCTGAAGCTGTTCCT |
a
Intron position is marked with a caret (^).
b
timeless primers were designed to anneal to different exons separated by an ∼2.5-kb intron.