TABLE 1.
Strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| SP6 | h−leu1-32 | Lab stock |
| NW309 | h+ leu1-32 nda3-KM311 | Lab stock |
| NW653 | h+ leu1-32 ura4D18 chk1∷ura4 cdc17-K42 ade6-216 | Lab stock |
| NW731 | h+ leu1-32 msc1∷kanR cdc25-22 | Lab stock |
| NW1469 | h−leu1-32 mis12-HA∷LEU2 | Mitsuhiro Yanagida |
| NW1525 | h−leu1-32 ura4D18 cnp1∷ura4 lys1+∷cnp1-1 | Mitsuhiro Yanagida |
| NW1526 | h−leu1-32 lys1+∷cnp1-GFP | Kohta Takahashi |
| NW1564 | h−leu1-32 msc1∷kanR | This study |
| NW1580 | h−leu1-32 mis12-375 | Mitsuhiro Yanagida |
| NW1601 | h−leu1-32 msc1∷kanR nda3-KM311 | This study |
| NW1608 | h−leu1-32 msc1-myc∷kanR | This study |
| NW1617 | h+ leu1-32 mis12-375 msc1∷kanR | This study |
| NW1698 | h−leu1-32 ura4D18 cdc25-22 | This study |
| NW1699 | h+ leu1-32 ura4D18 cdc25-22 msc1∷kanR | This study |
| NW1700 | h+ leu1-32 ura4D18 cdc25-22 mad2∷ura4 ade6-210 | Matthew O'Connell |
| NW1702 | h−leu1-32 mis6-302 | Mitsuhiro Yanagida |
| NW1703 | h+ leu1-32 mis6-302 msc1∷kanR ade6-210 | This study |
| NW1704 | h+ leu1-32 ura4D18 mad2∷ura4 chk1∷ura4 cdc17-K42 ade6-216 | This study |
| NW1713 | h−leu1-32 ura4D18 mad2∷ura4 chk1∷ura4 ade6-216 | This study |
| NW1718 | h−leu1-32 ura4D18 mad2∷ura4 cdc17-K42 ade6-210 | This study |
| NW1736 | h+ leu1-32 ade6-210 ura4D18 msc1∷kanR cnp1∷ura4 lys1+∷cnp1-1 | This study |
| NW1737 | h−leu1-32 lys1+∷cnp1-GFP msc1∷kanR | This study |
| NW1800 | h+ leu1-32 ade6-210 msc1∷kanR | This study |
| NW1828 | h+ leu1-32 ade6-210 ura4D18 pht1∷ura4 | Antony Carr |
| NW1829 | h+ leu1-32 ade6-210 ura4D18 pht1∷ura4 msc1∷kanR | This study |
| NW1830 | h−leu1-32 ade6-210 ura4D18 mis6-302 pht1∷ura4 msc1∷kanR | This study |
| NW1831 | h−leu1-32 ura4D18 mis12-375 pht1∷ura4 | This study |
| NW1832 | h+ leu1-32 ura4D18 mis12-375 pht1∷ura4 msc1∷kanR | This study |
| NW1833 | h−leu1-32 ade6-210 ura4D18 pht1∷ura4 cdc17-K42 | This study |
| NW1834 | h+ leu1-32 ade6-210 ura4D18 arg3-D4 pht1∷ura4 chk1∷arg3 cdc17-K42 | This study |
| NW1835 | h+ leu1-32 ade6-210 arg3-D4 chk1∷arg3 cdc17-K42 | This study |
| NW1836 | h−leu1-32 ade6-210 ura4D18 mis6-302 cnp1∷ura4 lys1+∷cnp1-1 | This study |
| NW1837 | h+ leu1-32 ade6-210 ura4D18 pht1∷ura4 cnp1∷ura4 lys1+∷cnp1-1 | This study |
| NW1839 | h+ leu1-32 ade6-210 ura4D18 pht1∷ura4 msc1∷kanR cnp1∷ura4 lys1+∷cnp1-1 | This study |
| NW1854 | h+ ade6-210 (Ch16 ade6-216) leu1-32 | Chris Norbury |
| NW1855 | h+ ade6-210 (Ch16 ade6-216) msc1∷kanR leu1-32 | This study |
| NW1856 | h+ ade6-210 (Ch16 ade6-216) pht1∷ura4 leu1-32 ura4D18 | This study |
| NW1857 | h+ ade6-210 (Ch16 ade6-216) msc1∷kanR pht1∷ura4 leu1-32 ura4D18 | This study |