Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Nov;160(2):778–780. doi: 10.1128/jb.160.2.778-780.1984

Initial reactions involved in the dissimilation of mandelate by Rhodotorula graminis.

D R Durham
PMCID: PMC214804  PMID: 6389497

Abstract

Rhodotorula graminis utilized DL-mandelate, L(+)-mandelate, and D(-)-mandelate as sole sources of carbon and energy. Growth on these aromatic substrates resulted in the induction of an NAD-dependent D(-)-mandelate dehydrogenase and a dye-linked L(+)-mandelate dehydrogenase, each catalyzing the stereospecific conversion of its respective enantiomer of mandelate to benzoylformate. Benzoylformate was oxidized to benzaldehyde, which was dehydrogenated to benzoate by an NAD-dependent benzaldehyde dehydrogenase. Benzoate was further metabolized through p-hydroxybenzoate and the protocatechuate branch of the beta-ketoadipate pathway.

Full text

PDF
778

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Durham D. R., McNamee C. G., Stewart D. B. Dissimilation of aromatic compounds in Rhodotorula graminis: biochemical characterization of pleiotropically negative mutants. J Bacteriol. 1984 Nov;160(2):771–777. doi: 10.1128/jb.160.2.771-777.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Durham D. R., Perry J. J. Purification and characterization of a heme-containing amine dehydrogenase from Pseudomonas putida. J Bacteriol. 1978 Jun;134(3):837–843. doi: 10.1128/jb.134.3.837-843.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Durham D. R., Phibbs P. V., Jr Fractionation and characterization of the phosphoenolpyruvate: fructose 1-phosphotransferase system from Pseudomonas aeruginosa. J Bacteriol. 1982 Feb;149(2):534–541. doi: 10.1128/jb.149.2.534-541.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Durham D. R., Stirling L. A., Ornston L. N., Perry J. J. Intergeneric evolutionary homology revealed by the study of protocatechuate 3,4-dioxygenase from Azotobacter vinelandii. Biochemistry. 1980 Jan 8;19(1):149–155. doi: 10.1021/bi00542a023. [DOI] [PubMed] [Google Scholar]
  5. Fee J. A., Hegeman G. D., Kenyon G. L. Mandelate racemase from Pseudomonas putida. Subunit composition and absolute divalent metal ion requirement. Biochemistry. 1974 Jun 4;13(12):2528–2532. doi: 10.1021/bi00709a008. [DOI] [PubMed] [Google Scholar]
  6. GUNSALUS C. F., STANIER R. Y., GUNSALUS I. C. The enzymatic conversion of mandelic acid to benzoic acid. III. Fractionation and properties of the soluble enzymes. J Bacteriol. 1953 Nov;66(5):548–553. doi: 10.1128/jb.66.5.548-553.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Halpin R. A., Hegeman G. D., Kenyon G. L. Carbon-13 nuclear magnetic resonance studies of mandelate metabolism in whole bacterial cells and in isolated, in vivo cross-linked enzyme complexes. Biochemistry. 1981 Mar 17;20(6):1525–1533. doi: 10.1021/bi00509a018. [DOI] [PubMed] [Google Scholar]
  8. Hegeman G. D., Rosenberg E. Y., Kenyon G. L. Mandelic acid racemase from Pseudomonas putida. Purification and properties of the enzyme. Biochemistry. 1970 Oct 13;9(21):4029–4036. doi: 10.1021/bi00823a001. [DOI] [PubMed] [Google Scholar]
  9. Hegeman G. D. Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type. J Bacteriol. 1966 Mar;91(3):1140–1154. doi: 10.1128/jb.91.3.1140-1154.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hegeman G. D. Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. II. Isolation and properties of blocked mutants. J Bacteriol. 1966 Mar;91(3):1155–1160. doi: 10.1128/jb.91.3.1155-1160.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jamaluddin M., Rao P. V., Vaidyanathan C. S. Involvement of the protocatechuate pathway in the metabolism of mandelic acid by Aspergillus niger. J Bacteriol. 1970 Mar;101(3):786–793. doi: 10.1128/jb.101.3.786-793.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kennedy S. I., Fewson C. A. Metabolism of mandelate and related compounds by bacterium NCIB 8250. J Gen Microbiol. 1968 Sep;53(2):259–273. doi: 10.1099/00221287-53-2-259. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. STANIER R. Y., GUNSALUS I. C., GUNSALUS C. F. The enzymatic conversion of mandelic acid to benzoic acid. II. Properties of the particulate fractions. J Bacteriol. 1953 Nov;66(5):543–547. doi: 10.1128/jb.66.5.543-547.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES