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. 1984 Nov;160(2):815–817. doi: 10.1128/jb.160.2.815-817.1984

Integration of plasmid DNA coding for beta-lactamase production in the Haemophilus influenzae chromosome.

M Murphey-Corb, M Nolan-Willard, R S Daum
PMCID: PMC214815  PMID: 6094493

Abstract

Of beta-lactamase-producing strains of Haemophilus influenzae, 65% do not contain extrachromosomal plasmid DNA. These strains, however, conjugally transfer beta-lactamase production to a recipient strain from which a 30-megadalton plasmid can be isolated. Restriction enzyme analysis and Southern transfer of DNA from both donor and recipient strains revealed that chromosomal integration of plasmid sequences occurred in all donor strains examined.

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Selected References

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  1. Anderson P., Johnston R. B., Jr, Smith D. H. Human serum activities against Hemophilus influenzae, type b. J Clin Invest. 1972 Jan;51(1):31–38. doi: 10.1172/JCI106793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bendler J. W., 3rd Physical size of the donor locus and transmission of Haemophilus influenzae ampicillin resistance genes by deoxyribonucleic acid-mediated transformation. J Bacteriol. 1976 Jan;125(1):197–204. doi: 10.1128/jb.125.1.197-204.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen J. C., Shank P. R., Morris V. L., Cardiff R., Varmus H. E. Integration of the DNA of mouse mammary tumor virus in virus-infected normal and neoplastic tissue of the mouse. Cell. 1979 Feb;16(2):333–345. doi: 10.1016/0092-8674(79)90010-2. [DOI] [PubMed] [Google Scholar]
  4. Daum R. S., Syriopoulou V. P., Smith A. L., Scheifele D. W., Willard J. E. Loss of plasmid DNA coding for beta-lactamase during experimental infection with Haemophilus influenzae type b. J Infect Dis. 1981 Apr;143(4):548–553. doi: 10.1093/infdis/143.4.548. [DOI] [PubMed] [Google Scholar]
  5. De Graaff J., Elwell L. P., Falkow S. Molecular nature of two beta-lactamase-specifying plasmids isolated from Haemophilus influenzae type b. J Bacteriol. 1976 Apr;126(1):439–446. doi: 10.1128/jb.126.1.439-446.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elwell L. P., De Graaff J., Seibert D., Falkow S. Plasmid-linked ampicillin resistance in haempohilus influenza type b. Infect Immun. 1975 Aug;12(2):404–410. doi: 10.1128/iai.12.2.404-410.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Galas D. J., Chandler M. Structure and stability of Tn9-mediated cointegrates. Evidence for two pathways of transposition. J Mol Biol. 1982 Jan 15;154(2):245–272. doi: 10.1016/0022-2836(82)90063-8. [DOI] [PubMed] [Google Scholar]
  8. Grindley N. D., Joyce C. M. Analysis of the structure and function of the kanamycin-resistance transposon Tn903. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):125–133. doi: 10.1101/sqb.1981.045.01.021. [DOI] [PubMed] [Google Scholar]
  9. Grindley N. D. Transposition of Tn3 and related transposons. Cell. 1983 Jan;32(1):3–5. doi: 10.1016/0092-8674(83)90490-7. [DOI] [PubMed] [Google Scholar]
  10. Hirschel B. J., Galas D. J., Chandler M. Cointegrate formation by Tn5, but not transposition, is dependent on recA. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4530–4534. doi: 10.1073/pnas.79.15.4530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
  12. Lam S., Roth J. R. IS200: a Salmonella-specific insertion sequence. Cell. 1983 Oct;34(3):951–960. doi: 10.1016/0092-8674(83)90552-4. [DOI] [PubMed] [Google Scholar]
  13. Laufs R., Kaulfers P. M. Molecular characterization of a plasmid specifying ampicillin resistance and its relationship to other R factors from Haemophilus influenzae. J Gen Microbiol. 1977 Dec;103(2):277–286. doi: 10.1099/00221287-103-2-277. [DOI] [PubMed] [Google Scholar]
  14. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  15. Stuy J. H. Plasmid transfer in Haemophilus influenzae. J Bacteriol. 1979 Aug;139(2):520–529. doi: 10.1128/jb.139.2.520-529.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  17. Willard J. E., Johnson E. J., Daum R. S. Interrelationships between physical state, phenotypic stability and transferability of beta-lactamase genes in Haemophilus influenzae. J Gen Microbiol. 1982 Oct;128(10):2353–2360. doi: 10.1099/00221287-128-10-2353. [DOI] [PubMed] [Google Scholar]

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