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. 1985 Jan;161(1):47–50. doi: 10.1128/jb.161.1.47-50.1985

Germination-initiated spores of Bacillus brevis Nagano retain their resistance properties.

E Daher, E Rosenberg, A L Demain
PMCID: PMC214833  PMID: 2578449

Abstract

Initiated spores and vegetative cells of the gramicidin S-producing Bacillus brevis Nagano were compared with respect to their resistance to various forms of stress (osmotic shock-starvation, exposure to ethanol, sonic oscillation, and heat). The resistance of initiated spores to all of these stress situations was considerably greater than that of vegetative cells and approached that of dormant spores. The period during which the initiated spores remained resistant to heat was extended by addition of gramicidin S. The antibiotic may therefore be of survival value to the species in nature by slowing down the development of initiated spores in the outgrowth phase of germination, thereby extending the period during which the cells are resistant to environmental stress.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DEMAIN A. L. Minimal media for quantitative studies with Bacillus subtilis. J Bacteriol. 1958 May;75(5):517–522. doi: 10.1128/jb.75.5.517-522.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dring G. J., Gould G. W. Sequence of events during rapid germination of spores of Bacillus cereus. J Gen Microbiol. 1971 Jan;65(1):101–104. doi: 10.1099/00221287-65-1-101. [DOI] [PubMed] [Google Scholar]
  3. Egorov N. S., Vypiiach A. N., Zharikova G. G., Maksimov V. N. Vliianie razlichnykh faktorov na prorastanie spor u variantov S i P- Bacillus brevis. Mikrobiologiia. 1975 Mar-Apr;44(2):237–240. [PubMed] [Google Scholar]
  4. Groves M. J. Some aggregation effects observed with an emulsion dispersed in saline containing cetrimide. J Pharm Pharmacol. 1966 May;18(5):305–311. doi: 10.1111/j.2042-7158.1966.tb07876.x. [DOI] [PubMed] [Google Scholar]
  5. Hsieh L. K., Vary J. C. Germination and peptidoglycan solubilization in Bacillus megaterium spores. J Bacteriol. 1975 Aug;123(2):463–470. doi: 10.1128/jb.123.2.463-470.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kambe M., Imae Y., Kurahashi K. Biochemical studies on gramicidin S non-producing mutants of Bacillus brevis ATCC 9999. J Biochem. 1974 Mar;75(3):481–493. doi: 10.1093/oxfordjournals.jbchem.a130417. [DOI] [PubMed] [Google Scholar]
  7. Levinson H. S., Hyatt M. T. Sequence of events during Bacillus megaterim spore germination. J Bacteriol. 1966 May;91(5):1811–1818. doi: 10.1128/jb.91.5.1811-1818.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Marahiel M. A., Danders W., Krause M., Kleinkauf H. Biological role of gramicidin S in spore functions. Studies on gramicidin-S-negative mutants of Bacillus brevis ATCC9999. Eur J Biochem. 1979 Aug 15;99(1):49–55. doi: 10.1111/j.1432-1033.1979.tb13229.x. [DOI] [PubMed] [Google Scholar]
  9. Nandi S., Seddon B. Evidence for gramicidin S functioning as a bacterial hormone specifically regulating spore outgrowth in Bacillus brevis Nagano [proceedings]. Biochem Soc Trans. 1978;6(2):409–411. doi: 10.1042/bst0060409. [DOI] [PubMed] [Google Scholar]
  10. POWELL J. F. Factors affecting the germination of thick suspensions of bacillus subtilis spores in L-alanine solution. J Gen Microbiol. 1950 Sep;4(3):330–338. doi: 10.1099/00221287-4-3-330. [DOI] [PubMed] [Google Scholar]
  11. Pandey N. K., Gollakota K. G. Induction of microcycle sporulation in Bacillus brevis spp. AG4 by amethopterin. Arch Microbiol. 1977 Aug 26;114(2):189–191. doi: 10.1007/BF00410783. [DOI] [PubMed] [Google Scholar]
  12. Piret J. M., Demain A. L. Germination initiation and outgrowth of spores of Bacillus brevis strain Nagano and its gramicidin S-negative mutant. Arch Microbiol. 1982 Nov;133(1):38–43. doi: 10.1007/BF00943767. [DOI] [PubMed] [Google Scholar]
  13. Piret J. M., Demain A. L. Sporulation and spore properties of Bacillus brevis and its gramicidin S-negative mutant. J Gen Microbiol. 1983 May;129(5):1309–1316. doi: 10.1099/00221287-129-5-1309. [DOI] [PubMed] [Google Scholar]
  14. Rossignol D. P., Vary J. C. Biochemistry of L-proline-triggered germination of Bacillus megaterium spores. J Bacteriol. 1979 May;138(2):431–441. doi: 10.1128/jb.138.2.431-441.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sarkar N., Paulus H. Function of peptide antibiotics in sporulation. Nat New Biol. 1972 Oct 25;239(95):228–230. doi: 10.1038/newbio239228a0. [DOI] [PubMed] [Google Scholar]
  16. Schaeffer P. Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriol Rev. 1969 Mar;33(1):48–71. doi: 10.1128/br.33.1.48-71.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Setlow P., Primus G. Protein metabolism during germination of Bacillus megaterium spores. I. Protein synthesis and amino acid metabolism. J Biol Chem. 1975 Jan 25;250(2):623–630. [PubMed] [Google Scholar]
  18. Setlow P. Purification and properties of some unique low molecular weight basic proteins degraded during germination of Bacillus megaterium spores. J Biol Chem. 1975 Oct 25;250(20):8168–8173. [PubMed] [Google Scholar]
  19. Seto-Young D. L., Ellar D. J. Membrane changes during germination of Bacillus megaterium KM spores. Microbios. 1979;26(103):7–15. [PubMed] [Google Scholar]
  20. Symons D. C., Hodgson B. Isolation and properties of Bacillus brevis mutants unable to produce tyrocidine. J Bacteriol. 1982 Aug;151(2):580–590. doi: 10.1128/jb.151.2.580-590.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vinter V., Slepecky R. A. Direct Transition of Outgrowing Bacterial Spores to New Sporangia Without Intermediate Cell Division. J Bacteriol. 1965 Sep;90(3):803–807. doi: 10.1128/jb.90.3.803-807.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

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