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. 1985 Jan;161(1):91–95. doi: 10.1128/jb.161.1.91-95.1985

Generation of transducing particles in Staphylococcus aureus.

D W Dyer, M I Rock, C Y Lee, J J Iandolo
PMCID: PMC214839  PMID: 3155719

Abstract

Transduction of plasmid pC194 and bacteriophage phi 11de varied inversely with the multiplicity of infection. As the multiplicity of infection decreased from 10(-1) to 10(-5) PFU/CFU, the transduction frequency of pC194 increased 10(4)-fold; the transduction frequency of phi 11de increased 300-fold with a 100-fold decrease in multiplicity of infection. Physical and genetic analysis of the transduced DNA showed that pC194 resided in the phage particle as a random, circularly permuted linear concatemer. In DNA prepared from phage that cotransduced pC194 and phi 11de, pC194 resided in the transducing phage primarily as a linear multimer of 15.8 kilobases, or about 5.4 pC194 monomers. The pC194 multimer was randomly inserted into the phi 11 genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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