Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 Jan;161(1):417–424. doi: 10.1128/jb.161.1.417-424.1985

Identification of pKM101-encoded loci specifying potentially lethal gene products.

S C Winans, G C Walker
PMCID: PMC214887  PMID: 3881396

Abstract

Two pKM101-encoded loci (designated kilA and kilB) have been identified which elaborate products that are potentially lethal to the bacterial cell. The lethal effects of each of these products is inhibited by two other plasmid-encoded loci, designated korA and korB (for kil override). Both korA and korB are required to control the lethality of either kil gene. In the presence of korA and korB both kil genes have other phenotypes: kilB is necessary for conjugal transfer, whereas kilA is responsible for the small-colony morphology on defined media that is characteristic of pKM101-containing strains (the Slo phenotype).

Full text

PDF
417

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bechhofer D. H., Figurski D. H. Map location and nucleotide sequence of korA, a key regulatory gene of promiscuous plasmid RK2. Nucleic Acids Res. 1983 Nov 11;11(21):7453–7469. doi: 10.1093/nar/11.21.7453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berg D. E., Weiss A., Crossland L. Polarity of Tn5 insertion mutations in Escherichia coli. J Bacteriol. 1980 May;142(2):439–446. doi: 10.1128/jb.142.2.439-446.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradley D. E., Rutherford E. L. Basic characterization of a lipid-containing bacteriophage specific for plasmids of the P, N, and W compatibility groups. Can J Microbiol. 1975 Feb;21(2):152–163. doi: 10.1139/m75-023. [DOI] [PubMed] [Google Scholar]
  4. Bradley D. E., Taylor D. E., Cohen D. R. Specification of surface mating systems among conjugative drug resistance plasmids in Escherichia coli K-12. J Bacteriol. 1980 Sep;143(3):1466–1470. doi: 10.1128/jb.143.3.1466-1470.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown A. M., Willetts N. S. A physical and genetic map of the IncN plasmid R46. Plasmid. 1981 Mar;5(2):188–201. doi: 10.1016/0147-619x(81)90020-2. [DOI] [PubMed] [Google Scholar]
  6. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chernin L. S., Mikoyan V. S. Effects of plasmids on chromosome metabolism in bacteria. Plasmid. 1981 Jul;6(1):119–140. doi: 10.1016/0147-619x(81)90059-7. [DOI] [PubMed] [Google Scholar]
  8. Datta N., Hedges R. W. Host ranges of R factors. J Gen Microbiol. 1972 May;70(3):453–460. doi: 10.1099/00221287-70-3-453. [DOI] [PubMed] [Google Scholar]
  9. Elledge S. J., Walker G. C. Proteins required for ultraviolet light and chemical mutagenesis. Identification of the products of the umuC locus of Escherichia coli. J Mol Biol. 1983 Feb 25;164(2):175–192. doi: 10.1016/0022-2836(83)90074-8. [DOI] [PubMed] [Google Scholar]
  10. Figurski D. H., Pohlman R. F., Bechhofer D. H., Prince A. S., Kelton C. A. Broad host range plasmid RK2 encodes multiple kil genes potentially lethal to Escherichia coli host cells. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1935–1939. doi: 10.1073/pnas.79.6.1935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Langer P. J., Shanabruch W. G., Walker G. C. Functional organization of plasmid pKM101. J Bacteriol. 1981 Mar;145(3):1310–1316. doi: 10.1128/jb.145.3.1310-1316.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Langer P. J., Walker G. C. Restriction endonuclease cleavage map of pKM101: relationship to parental plasmid R46. Mol Gen Genet. 1981;182(2):268–272. doi: 10.1007/BF00269669. [DOI] [PubMed] [Google Scholar]
  13. McCann J., Spingarn N. E., Kobori J., Ames B. N. Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids. Proc Natl Acad Sci U S A. 1975 Mar;72(3):979–983. doi: 10.1073/pnas.72.3.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Meacock P. A., Cohen S. N. Genetic analysis of the inter-relationship between plasmid replication and incompatibility. Mol Gen Genet. 1979 Jul 13;174(2):135–147. doi: 10.1007/BF00268351. [DOI] [PubMed] [Google Scholar]
  15. Mortelmans K. E., Stocker B. A. Segregation of the mutator property of plasmid R46 from its ultraviolet-protecting property. Mol Gen Genet. 1979 Jan 2;167(3):317–327. doi: 10.1007/BF00267425. [DOI] [PubMed] [Google Scholar]
  16. Mulligan J. T., Margolin W., Krueger J. H., Walker G. C. Mutations affecting regulation of methionine biosynthetic genes isolated by use of met-lac fusions. J Bacteriol. 1982 Aug;151(2):609–619. doi: 10.1128/jb.151.2.609-619.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ogura T., Hiraga S. Mini-F plasmid genes that couple host cell division to plasmid proliferation. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4784–4788. doi: 10.1073/pnas.80.15.4784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Perry K. L., Walker G. C. Identification of plasmid (pKM101)-coded proteins involved in mutagenesis and UV resistance. Nature. 1982 Nov 18;300(5889):278–281. doi: 10.1038/300278a0. [DOI] [PubMed] [Google Scholar]
  19. Rambach A., Hogness D. S. Translation of Drosophila melanogaster sequences in Escherichia coli. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5041–5045. doi: 10.1073/pnas.74.11.5041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rodriguez M., Iyer V. N. Killing of Klebsiella pneumoniae mediated by conjugation with bacteria carrying antibiotic-resistance plasmids of the group N. Plasmid. 1981 Jul;6(1):141–147. doi: 10.1016/0147-619x(81)90060-3. [DOI] [PubMed] [Google Scholar]
  21. Walker G. C. Plasmid (pKM101)-mediated enhancement of repair and mutagenesis: dependence on chromosomal genes in Escherichia coli K-12. Mol Gen Genet. 1977 Mar 28;152(1):93–103. doi: 10.1007/BF00264945. [DOI] [PubMed] [Google Scholar]
  22. Winans S. C., Walker G. C. Conjugal transfer system of the IncN plasmid pKM101. J Bacteriol. 1985 Jan;161(1):402–410. doi: 10.1128/jb.161.1.402-410.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Winans S. C., Walker G. C. Entry exclusion determinant(s) of IncN plasmid pKM101. J Bacteriol. 1985 Jan;161(1):411–416. doi: 10.1128/jb.161.1.411-416.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Winans S. C., Walker G. C. Genetic localization and characterization of a pKM101-coded endonuclease. J Bacteriol. 1983 Jun;154(3):1117–1125. doi: 10.1128/jb.154.3.1117-1125.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Young C., Bechhofer D. H., Figurski D. H. Gene regulation in plasmid RK2: positive control by korA in the expression of korC. J Bacteriol. 1984 Jan;157(1):247–252. doi: 10.1128/jb.157.1.247-252.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES