Skip to main content
NCBI home page
The British Journal of Cancer. Supplement logoLink to The British Journal of Cancer. Supplement
. 1973 Aug;1:142–152.

Significance of Tumour Associated Antigens on Human Colonic Carcinomata

M J Embleton
PMCID: PMC2149074  PMID: 4804277

Abstract

A microcytotoxicity assay was used to detect cell mediated immunity against colon and rectum carcinoma cells in vitro. Lymphocytes from 62% of colon carcinoma patients were cytotoxic for the cells, but no cross reactions between colon carcinomata and tumours of other types were observed.

The effect of papain solubilized tumour membrane extracts was evaluated by testing lymphocytes for reactivity following preincubation with the extracts. Soluble preparations of pooled colon carcinomata inhibited cytotoxicity by sensitized lymphocytes, but similar extracts of normal colon or melanoma had no inhibitory effect. The results suggest that soluble tumour antigen may play a role in abolishing lymphocyte reactivity, and this supports the concept that cellular immunity against tumours in vivo may be inhibited by circulating antigen.

Full text

PDF
142

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baldwin R. W., Embleton M. J., Robins R. A. Cellular and humoral immunity to rat hepatoma-specific antigens correlated with tumour status. Int J Cancer. 1973 Jan 15;11(1):1–10. doi: 10.1002/ijc.2910110102. [DOI] [PubMed] [Google Scholar]
  2. Baldwin R. W., Harris J. R., Price M. R. Fractionation of plasma membrane-associated tumor-specific antigen from an aminoazo dye-induced rat hepatoma. Int J Cancer. 1973 Mar 15;11(2):385–397. doi: 10.1002/ijc.2910110216. [DOI] [PubMed] [Google Scholar]
  3. Baldwin R. W., Price M. R., Robins R. A. Blocking of lymphocyte-mediated cytotoxicity for rat hepatoma cells by tumour-specific antigen-antibody complexes. Nat New Biol. 1972 Aug 9;238(84):185–186. doi: 10.1038/newbio238185a0. [DOI] [PubMed] [Google Scholar]
  4. Baldwin R. W., Price M. R., Robins R. A. Inhibition of hepatoma-immune lymph-node cell cytotoxicity by tumour-bearer serum, and solubilized hepatoma antigen. Int J Cancer. 1973 May;11(3):527–535. doi: 10.1002/ijc.2910110304. [DOI] [PubMed] [Google Scholar]
  5. Bubeník J., Perlmann P., Helmstein K., Moberger G. Cellular and humoral immune responses to human urinary bladder carcinomas. Int J Cancer. 1970 May 15;5(3):310–319. doi: 10.1002/ijc.2910050303. [DOI] [PubMed] [Google Scholar]
  6. Currie G. A., Basham C. Serum mediated inhibition of the immunological reactions of the patient to his own tumour: a possible role for circulating antigen. Br J Cancer. 1972 Dec;26(6):427–438. doi: 10.1038/bjc.1972.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GOLD P., FREEDMAN S. O. DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES. J Exp Med. 1965 Mar 1;121:439–462. doi: 10.1084/jem.121.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gold P., Freedman S. O. Specific carcinoembryonic antigens of the human digestive system. J Exp Med. 1965 Sep 1;122(3):467–481. doi: 10.1084/jem.122.3.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hellström I., Hellström K. E., Evans C. A., Heppner G. H., Pierce G. E., Yang J. P. Serum-mediated protection of neoplastic cells from inhibition by lymphocytes immune to their tumor-specific antigens. Proc Natl Acad Sci U S A. 1969 Feb;62(2):362–368. doi: 10.1073/pnas.62.2.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hollinshead A. C., McWright C. G., Alford TC GLEW D. H., Gold P., Herbeman R. B. Separation of skin reactive intestinal cancer antigen from the carcinoembryonic antigen of Gold. Science. 1972 Sep 8;177(4052):887–889. doi: 10.1126/science.177.4052.887. [DOI] [PubMed] [Google Scholar]
  11. Hollinshead A., Glew D., Bunnag B., Gold P., Herberman R. Skin-reactive soluble antigen from intestinal cancer-cell-membranes and relationship to carcinoembryonic antigens. Lancet. 1970 Jun 6;1(7658):1191–1195. doi: 10.1016/s0140-6736(70)91784-8. [DOI] [PubMed] [Google Scholar]
  12. Krupey J., Gold P., Freedman S. O. Physicochemical studies of the carcinoembryonic antigens of the human digestive system. J Exp Med. 1968 Sep 1;128(3):387–398. doi: 10.1084/jem.128.3.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Laurence D. J., Neville A. M. Foetal antigens and their role in the diagnosis and clinical management of human neoplasms: a review. Br J Cancer. 1972 Oct;26(5):335–355. doi: 10.1038/bjc.1972.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nairn R. C., Nind A. P., Guli E. P., Davies D. J., Rolland J. M., McGiven A. R., Hughes E. S. Immunological reactivity in patients with carcinoma of colon. Br Med J. 1971 Dec 18;4(5789):706–709. doi: 10.1136/bmj.4.5789.706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sjögren H. O., Hellström I., Bansal S. C., Hellström K. E. Suggestive evidence that the "blocking antibodies" of tumor-bearing individuals may be antigen--antibody complexes. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1372–1375. doi: 10.1073/pnas.68.6.1372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sjögren H. O., Hellström I., Bansal S. C., Warner G. A., Hellström K. E. Elution of "blocking factors" from human tumors, capable of abrogating tumor-cell destruction by specifically immune lymphocytes. Int J Cancer. 1972 Mar 15;9(2):274–283. doi: 10.1002/ijc.2910090205. [DOI] [PubMed] [Google Scholar]
  18. Thomson D. M., Krupey J., Freedman S. O., Gold P. The radioimmunoassay of circulating carcinoembryonic antigen of the human digestive system. Proc Natl Acad Sci U S A. 1969 Sep;64(1):161–167. doi: 10.1073/pnas.64.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The British Journal of Cancer. Supplement are provided here courtesy of Cancer Research UK

RESOURCES