Abstract
The effect of ionizing radiation on the growth of bone marrow cells from haematologically normal controls or from patients with acute nonlymphocytic leukaemia was studied using colony formation as an endpoint. A modified agar culture method which incorporated daily feeding with new medium was utilized to allow the growth of leukaemic cell colonies. Analysis of radiation-dose survival curves revealed that normal bone marrow cell populations exhibited a relatively steep slope, with values of D0 averaging 0.88 Gy, and essentially no shoulder to the survival curves. The leukaemic cells tested also displayed survival curves with negligible shoulders and steep slopes (average value of D0 = 0.66 Gy). The average values of D0 for normal and leukaemic cells were significantly different (P less than 0.001). The ability of hematopoietic progenitor cells to recover from the accumulation of sublethal damage was also investigated by allowing a fractionation interval between doses. As expected from the lack of significant shoulder on the survival curve, there was no evidence of recovery from sublethal damage. These results provide a rationale for the use of fractionated radiation therapy for whole-body irradiation prior to bone marrow transplant.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bromer R. H., Mitchell J. B., Soares N. Response of human hematopoietic precursor cells (CFUc) to hyperthermia and radiation. Cancer Res. 1982 Apr;42(4):1261–1265. [PubMed] [Google Scholar]
- Broxmeyer H. E., Galbraith P. R., Baker F. L. Relationship of colony-stimulating activity to apparent kill of human colony-forming cells by irradiation and hydroxyurea. Blood. 1976 Mar;47(3):403–411. [PubMed] [Google Scholar]
- Grilli G., Nothdurft W., Fliedner T. M. Radiation sensitivity of human erythropoietic and granulopoietic progenitor cells in the blood and in the bone marrow. Int J Radiat Biol Relat Stud Phys Chem Med. 1982 Jun;41(6):685–687. doi: 10.1080/09553008214550781. [DOI] [PubMed] [Google Scholar]
- Neumann H. A., Löhr G. W., Fauser A. A. Radiation sensitivity of pluripotent hemopoietic progenitors (CFUGEMM) derived from human bone marrow. Exp Hematol. 1981 Aug;9(7):742–744. [PubMed] [Google Scholar]
- Ozawa K., Miura Y., Suda T., Motoyoshi K., Takaku F. Radiation sensitivity of leukemic progenitor cells in acute nonlymphocytic leukemia. Cancer Res. 1983 May;43(5):2339–2341. [PubMed] [Google Scholar]
- Park C. H., Wiernik P. H., Morrison F. S., Amare M., Van Sloten K. V., Maloney T. R. Clinical correlations of leukemic clonogenic cell chemosensitivity assessed by in vitro continuous exposure to drugs. Cancer Res. 1983 May;43(5):2346–2349. [PubMed] [Google Scholar]
- Peters L. J., Withers H. R., Cundiff J. H., Dicke K. A. Radiobiological considerations in the use of total-body irradiation for bone-marrow transplantation. Radiology. 1979 Apr;131(1):243–247. doi: 10.1148/131.1.243. [DOI] [PubMed] [Google Scholar]
- Rickard K. A., Brown R., Kronenberg H. Radiation and the human agar colony forming cell. Pathology. 1974 Apr;6(2):169–181. doi: 10.3109/00313027409068981. [DOI] [PubMed] [Google Scholar]
- Senn J. S., McCulloch E. A. Radiation sensitivity of human bone marrow cells measured by a cell culture method. Blood. 1970 Jan;35(1):56–60. [PubMed] [Google Scholar]
- Song C. W., Kim T. H., Khan F. M., Kersey J. H., Levitt S. H. Radiobiological basis of total body irradiation with different dose rate and fractionation: repair capacity of hemopoietic cells. Int J Radiat Oncol Biol Phys. 1981 Dec;7(12):1695–1701. doi: 10.1016/0360-3016(81)90195-4. [DOI] [PubMed] [Google Scholar]
- TILL J. E., McCULLOCH E. A. Early repair processes in marrow cells irradiated and proliferating in vivo. Radiat Res. 1963 Jan;18:96–105. [PubMed] [Google Scholar]