Abstract
A fibroblast cell line (3T3) and normal rat peritoneal fluid cells were exposed in vitro to pulsed ultrasound from a diagnostic instrument (Smith-Kline "Ekoline 20"). We report here on ultrastructural changes in both cell types and on altered motility patterns in 3T3 fibroblasts. Abnormal motility was detectable 10 generations after exposure. X-irradiation and ultraviolet light elicited similar effects on cell motion. It is suggested that the cellular effects of diagnostic levels of ultrasound be further examined both in vitro and in vivo.
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- Kennedy A. R., Fox M., Murphy G., Little J. B. Relationship between x-ray exposure and malignant transformation in C3H 10T1/2 cells. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7262–7266. doi: 10.1073/pnas.77.12.7262. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liebeskind D., Bases R., Elequin F., Neubort S., Leifer R., Goldberg R., Koenigsberg M. Diagnostic ultrasound: effects on the DNA and growth patterns of animal cells. Radiology. 1979 Apr;131(1):177–184. doi: 10.1148/131.1.177. [DOI] [PubMed] [Google Scholar]
- Liebeskind D., Bases R., Koenigsberg M., Koss L., Raventos C. Morphological changes in the surface characteristics of cultured cells after exposure to diagnostic ultrasound. Radiology. 1981 Feb;138(2):419–423. doi: 10.1148/radiology.138.2.7455124. [DOI] [PubMed] [Google Scholar]
- Liebeskind D., Bases R., Mendez F., Elequin F., Koenigsberg M. Sister chromatid exchanges in human lymphocytes after exposure to diagnostic ultrasound. Science. 1979 Sep 21;205(4412):1273–1275. doi: 10.1126/science.472742. [DOI] [PubMed] [Google Scholar]
- Puvion E., Lange M. Functional significance of perichromatin granule accumulation induced by cadmium chloride in isolated rat liver cells. Exp Cell Res. 1980 Jul;128(1):47–58. doi: 10.1016/0014-4827(80)90385-7. [DOI] [PubMed] [Google Scholar]
- Smith H. S., Springer E. L., Hackett A. J. Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. Cancer Res. 1979 Feb;39(2 Pt 1):332–344. [PubMed] [Google Scholar]
- Wegner R. D., Obe G., Meyenburg M. Has diagnostic ultrasound mutagenic effects? Hum Genet. 1980;56(1):95–98. doi: 10.1007/BF00281576. [DOI] [PubMed] [Google Scholar]