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. 1978 Jun;3:168–172.

Cytotoxicity of radiosensitizers in multicell spheroids: combination treatment with hyperthermia.

R Sutherland, W Macfarlane
PMCID: PMC2149386  PMID: 277220

Abstract

Spheroids of EMT6 tumour cells were grown in spinner culture. As with V79 cell spheroids, the combination of 1.5mM misonidazole and hyperthermia at 42.5 degree C for 1 or 2 h cuased greater cytotoxicity than either heat or drug alone. Radioresistant hypoxic cells at the centre of the spheroids were eliminated but other cells were also killed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biaglow J. E., Durand R. E. The effects of nitrobenzene derivatives on oxygen utilization and radiation response of an in vitro tumor model. Radiat Res. 1976 Mar;65(3):529–539. [PubMed] [Google Scholar]
  2. Bleehen N. M., Honess D. J., Morgan J. E. Interaction of hyperthermia and the hypoxic cell sensitizer Ro-07-0582 on the EMT6 mouse tumour. Br J Cancer. 1977 Mar;35(3):299–306. doi: 10.1038/bjc.1977.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Durand R. E., Biaglow J. E. Letter: Modification of the radiation response of an in vitro tumour model by control of cellular respiration. Int J Radiat Biol Relat Stud Phys Chem Med. 1974 Dec;26(6):597–601. doi: 10.1080/09553007414551641. [DOI] [PubMed] [Google Scholar]
  4. Foster J. L., Conroy P. J., Searle A. J., Willson R. L. Metronidazole (Flagyl): characterization as a cytotoxic drug specific for hypoxic tumour cells. Br J Cancer. 1976 May;33(5):485–490. doi: 10.1038/bjc.1976.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gray A. J., Dische S., Adams G. E., Flockhart I. R., Foster J. L. Clinical testing of the radiosensitiser Ro-07-0582. I. Dose tolerance, serum and tumour concentrations. Clin Radiol. 1976 Apr;27(2):151–157. doi: 10.1016/s0009-9260(76)80137-7. [DOI] [PubMed] [Google Scholar]
  6. Hahn G. M. Metabolic aspects of the role of hyperthermia im mammalian cell inactivation and their possible relevance to cancer treatment. Cancer Res. 1974 Nov;34(11):3117–3123. [PubMed] [Google Scholar]
  7. Hall E. J., Biaglow J. Ro-07-0582 as a radiosensitizer and cytotoxic agent. Int J Radiat Oncol Biol Phys. 1977 May-Jun;2(5-6):521–530. doi: 10.1016/0360-3016(77)90163-8. [DOI] [PubMed] [Google Scholar]
  8. Kim S. H., Kim J. H., Hahn E. Q. Letter: Enhanced killing of hypoxic tumour cells by hyperthemia. Br J Radiol. 1975 Oct;48(574):872–874. doi: 10.1259/0007-1285-48-574-872-c. [DOI] [PubMed] [Google Scholar]
  9. Mohindra J. K., Rauth A. M. Increased cell killing by metronidazole and nitrofurazone of hypoxic compared to aerobic mammalian cells. Cancer Res. 1976 Mar;36(3):930–936. [PubMed] [Google Scholar]
  10. Moore B. A., Palcic B., Skarsgard L. D. Radiosensitizing and toxic effects on the 2-nitroimidazole Ro-07-0582 in hypoxic mammation cells. Radiat Res. 1976 Sep;67(3):459–473. [PubMed] [Google Scholar]
  11. Sridhar R., Koch C., Suterland R. Cytotoxicity of two nitroimidazole radiosensitizers in an in vitro tumor model. Int J Radiat Oncol Biol Phys. 1976 Nov-Dec;1(11-12):1149–1157. doi: 10.1016/0360-3016(76)90088-2. [DOI] [PubMed] [Google Scholar]
  12. Sridhar R., Sutherland R. Hyperthermic potentiation of cytotoxicity of Ro-07-0582 in multicell spheroids. Int J Radiat Oncol Biol Phys. 1977 May-Jun;2(5-6):531–535. doi: 10.1016/0360-3016(77)90164-x. [DOI] [PubMed] [Google Scholar]
  13. Stratford I. J., Adams G. E. Effect of hyperthermia on differential cytotoxicity of a hypoxic cell radiosensitizer, Ro-07-0582, on mammalian cells in vitro. Br J Cancer. 1977 Mar;35(3):307–313. doi: 10.1038/bjc.1977.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sutherland R. M., Durand R. E. Radiation response of multicell spheroids--an in vitro tumour model. Curr Top Radiat Res Q. 1976 Jan;11(1):87–139. [PubMed] [Google Scholar]
  15. Sutherland R. M., Durand R. E. Radiosensitization by nifuroxime of the hypoxic cells in an in vitro tumour model. Int J Radiat Biol Relat Stud Phys Chem Med. 1972 Dec;22(6):613–618. doi: 10.1080/09553007214551511. [DOI] [PubMed] [Google Scholar]
  16. Sutherland R. M. Selective chemotherapy of noncycling cells in an in vitro tumor model. Cancer Res. 1974 Dec;34(12):3501–3503. [PubMed] [Google Scholar]

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