Skip to main content
NCBI home page
The British Journal of Cancer. Supplement logoLink to The British Journal of Cancer. Supplement
. 1978 Jun;3:107–110.

Toxic and radiosensitizing effect of the 2-nitroimidazole misonidazole (Ro-07-0582) on murine CFU in vivo.

E O Pettersen
PMCID: PMC2149398  PMID: 277208

Abstract

Spleen colony-forming stem cells of the bone marrow of mice (CFU) have been used as an in vivo test system for toxic and radiosensitizing effects of misonidazole. The cells were irradiated in vivo in the donors before they were suspended and injected i.v. into recipients. Hyoxic conditions were achieved by killing the donors 20 min before irradiation. A drug dose in donors of 1.0 mg/g body wt (ca 1200 microgram/ml in serum) was found to be weakly toxic over an exposure time of 50 min under hypoxic conditions. The radiosensitizing effect at this concentration was found to be about the same as the full effect of oxygen.

Full text

PDF
107

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asquith J. C., Watts M. E., Patel K., Smithen C. E., Adams G. E. Electron affinic sensitization. V. Radiosensitization of hypoxic bacteria and mammalian cells in vitro by some nitroimidazoles and nitropyrazoles. Radiat Res. 1974 Oct;60(1):108–118. [PubMed] [Google Scholar]
  2. Brown J. M. Selective radiosensitization of the hypoxic cells of mouse tumors with the nitroimidazoles metronidazole and Ro 7-0582. Radiat Res. 1975 Dec;64(3):633–647. [PubMed] [Google Scholar]
  3. Denekamp J., Harris S. R. Tests of two electron-affinic radiosensitizers in vivo using regrowth of an experimental carcinoma. Radiat Res. 1975 Feb;61(2):191–203. [PubMed] [Google Scholar]
  4. Denekamp J., Harris S. R. The response of a transplantable tumor to fractionated irradiation. 1. X rays and the hypoxic cell radiosensitizer Ro-07-0582. Radiat Res. 1976 Apr;66(1):66–75. [PubMed] [Google Scholar]
  5. George K. C., Hirst D. G., McNally N. J. Effect of hyperthermia on cytotoxicity of the radiosensitizer Ro-07-0582 in a solid mouse tumour. Br J Cancer. 1977 Mar;35(3):372–375. doi: 10.1038/bjc.1977.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hall E. J., Roizin-Towle L. Hypoxic sensitizers: radiobiological studies at the cellular level. Radiology. 1975 Nov;117(2):453–457. doi: 10.1148/117.2.453. [DOI] [PubMed] [Google Scholar]
  7. Hendry J. H., Gilbert C. W., Howard A. K values and gain factors of fast neutrons and x-rays for a mammalian cell system (CFU)in vivo. Radiat Res. 1975 Mar;61(3):504–512. [PubMed] [Google Scholar]
  8. Hendry J. H. The f number of primary transplanted splenic colony-forming cells. Cell Tissue Kinet. 1971 May;4(3):217–223. doi: 10.1111/j.1365-2184.1971.tb01532.x. [DOI] [PubMed] [Google Scholar]
  9. Johnson R., Gomer C., Pearce J. An investigation of the radiosensitizing effects of Ro-07-0582 on hypoxic skin in primates. Int J Radiat Oncol Biol Phys. 1976 Jul-Aug;1(7-8):593–599. doi: 10.1016/0360-3016(76)90140-1. [DOI] [PubMed] [Google Scholar]
  10. Moore B. A., Palcic B., Skarsgard L. D. Radiosensitizing and toxic effects on the 2-nitroimidazole Ro-07-0582 in hypoxic mammation cells. Radiat Res. 1976 Sep;67(3):459–473. [PubMed] [Google Scholar]
  11. Peters L. J. Modification of the radiocurability of a syngeneic murine squamous carcinoma by its site of growth, by electron-affinic drugs, and by ICRF 159. Br J Radiol. 1976 Aug;49(584):708–715. doi: 10.1259/0007-1285-49-584-708. [DOI] [PubMed] [Google Scholar]
  12. Pettersen E. O., Bøyum A., Laane B. F. X-ray inactivation of murine bone marrow cells as measured by the spleen colony assay and the diffusion chamber technique. Radiat Res. 1974 Jun;58(3):409–416. [PubMed] [Google Scholar]
  13. Pettersen E. O. Radiosensitizing and toxic effects of the 2-nitroimidazole Ro-07-0582 in different phases of the cell cycle of extremely hypoxic human cells in vitro. Radiat Res. 1978 Jan;73(1):180–191. [PubMed] [Google Scholar]
  14. Sheldon P. W., Hill S. A., Foster J. L., Fowler J. F. Radiosensitization of C3H mouse mammary tumours using fractionated doses of x rays with the drug Ro-07-0582. Br J Radiol. 1976 Jan;49(577):76–80. doi: 10.1259/0007-1285-49-577-76. [DOI] [PubMed] [Google Scholar]
  15. Stratford I. J., Adams G. E. Effect of hyperthermia on differential cytotoxicity of a hypoxic cell radiosensitizer, Ro-07-0582, on mammalian cells in vitro. Br J Cancer. 1977 Mar;35(3):307–313. doi: 10.1038/bjc.1977.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Thomlinson R. H., Dische S., Gray A. J., Errington L. M. Clinical testing of the radiosensitiser Ro-07-0582. III. Response of tumours. Clin Radiol. 1976 Apr;27(2):167–174. doi: 10.1016/s0009-9260(76)80139-0. [DOI] [PubMed] [Google Scholar]

Articles from The British Journal of Cancer. Supplement are provided here courtesy of Cancer Research UK

RESOURCES