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. 1985 Feb;161(2):758–763. doi: 10.1128/jb.161.2.758-763.1985

Separate resistances to arsenate and arsenite (antimonate) encoded by the arsenical resistance operon of R factor R773.

C M Chen, H L Mobley, B P Rosen
PMCID: PMC214947  PMID: 3881408

Abstract

The arsenical resistance operon of R factor R773 was analyzed by subcloning and insertional inactivation. The operon was found to have two functional regions, the promoter-proximal region encoding resistance to arsenite and antimonate and the promoter-distal region encoding arsenate resistance. A unique 1.6-kilobase fragment was shown to be sufficient to encode arsenate resistance and produce arsenate extrusion from intact cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berg D. E., Weiss A., Crossland L. Polarity of Tn5 insertion mutations in Escherichia coli. J Bacteriol. 1980 May;142(2):439–446. doi: 10.1128/jb.142.2.439-446.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Butlin J. D., Cox G. B., Gibson F. Oxidative phosphorylation in Escherichia coli K12. Mutations affecting magnesium ion- or calcium ion-stimulated adenosine triphosphatase. Biochem J. 1971 Aug;124(1):75–81. doi: 10.1042/bj1240075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Götz F., Zabielski J., Philipson L., Lindberg M. DNA homology between the arsenate resistance plasmid pSX267 from Staphylococcus xylosus and the penicillinase plasmid pI258 from Staphylococcus aureus. Plasmid. 1983 Mar;9(2):126–137. doi: 10.1016/0147-619x(83)90015-x. [DOI] [PubMed] [Google Scholar]
  5. Hedges R. W., Baumberg S. Resistance to arsenic compounds conferred by a plasmid transmissible between strains of Escherichia coli. J Bacteriol. 1973 Jul;115(1):459–460. doi: 10.1128/jb.115.1.459-460.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hendrick C. A., Haskins W. P., Vidaver A. K. Conjugative Plasmid in Corynebacterium flaccumfaciens subsp. oortii That Confers Resistance to Arsenite, Arsenate, and Antimony(III). Appl Environ Microbiol. 1984 Jul;48(1):56–60. doi: 10.1128/aem.48.1.56-60.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jorgensen R. A., Rothstein S. J., Reznikoff W. S. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet. 1979;177(1):65–72. doi: 10.1007/BF00267254. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Mobley H. L., Chen C. M., Silver S., Rosen B. P. Cloning and expression of R-factor mediated arsenate resistance in Escherichia coli. Mol Gen Genet. 1983;191(3):421–426. doi: 10.1007/BF00425757. [DOI] [PubMed] [Google Scholar]
  11. Mobley H. L., Rosen B. P. Energetics of plasmid-mediated arsenate resistance in Escherichia coli. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6119–6122. doi: 10.1073/pnas.79.20.6119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mobley H. L., Silver S., Porter F. D., Rosen B. P. Homology among arsenate resistance determinants of R factors in Escherichia coli. Antimicrob Agents Chemother. 1984 Feb;25(2):157–161. doi: 10.1128/aac.25.2.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Novick R. P., Murphy E., Gryczan T. J., Baron E., Edelman I. Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps. Plasmid. 1979 Jan;2(1):109–129. doi: 10.1016/0147-619x(79)90010-6. [DOI] [PubMed] [Google Scholar]
  14. Novick R. P., Roth C. Plasmid-linked resistance to inorganic salts in Staphylococcus aureus. J Bacteriol. 1968 Apr;95(4):1335–1342. doi: 10.1128/jb.95.4.1335-1342.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Osborne F. H., Enrlich H. L. Oxidation of arsenite by a soil isolate of Alcaligenes. J Appl Bacteriol. 1976 Oct;41(2):295–305. doi: 10.1111/j.1365-2672.1976.tb00633.x. [DOI] [PubMed] [Google Scholar]
  16. Rosen B. P., Borbolla M. G. A plasmid-encoded arsenite pump produces arsenite resistance in Escherichia coli. Biochem Biophys Res Commun. 1984 Nov 14;124(3):760–765. doi: 10.1016/0006-291x(84)91023-4. [DOI] [PubMed] [Google Scholar]
  17. Silver S., Budd K., Leahy K. M., Shaw W. V., Hammond D., Novick R. P., Willsky G. R., Malamy M. H., Rosenberg H. Inducible plasmid-determined resistance to arsenate, arsenite, and antimony (III) in escherichia coli and Staphylococcus aureus. J Bacteriol. 1981 Jun;146(3):983–996. doi: 10.1128/jb.146.3.983-996.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Silver S., Keach D. Energy-dependent arsenate efflux: the mechanism of plasmid-mediated resistance. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6114–6118. doi: 10.1073/pnas.79.20.6114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stüber D., Bujard H. Organization of transcriptional signals in plasmids pBR322 and pACYC184. Proc Natl Acad Sci U S A. 1981 Jan;78(1):167–171. doi: 10.1073/pnas.78.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]

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