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. 1985 Feb;161(2):769–771. doi: 10.1128/jb.161.2.769-771.1985

Expression of a Saccharomyces cerevisiae photolyase gene in Escherichia coli.

G B Sancar
PMCID: PMC214950  PMID: 3881410

Abstract

A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a DNA photolyase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amann E., Brosius J., Ptashne M. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene. 1983 Nov;25(2-3):167–178. doi: 10.1016/0378-1119(83)90222-6. [DOI] [PubMed] [Google Scholar]
  2. Boatwright D. T., Madden J. J., Denson J., Werbin H. Yeast DNA photolyase: molecular weight, subunit structure, and reconstruction of active enzyme from its subunits. Biochemistry. 1975 Dec 16;14(25):5418–5421. doi: 10.1021/bi00696a006. [DOI] [PubMed] [Google Scholar]
  3. Harm W., Harm H., Rupert C. S. Analysis of photoenzymatic repair of UV lesions in DNA by single light flashes. II. In vivo studies with Escherichia coli cells and bacteriophage. Mutat Res. 1968 Nov-Dec;6(3):371–385. doi: 10.1016/0027-5107(68)90054-7. [DOI] [PubMed] [Google Scholar]
  4. Iwatsuki N., Joe C. O., Werbin H. Evidence that deoxyribonucleic acid photolyase from baker's yeast is a flavoprotein. Biochemistry. 1980 Mar 18;19(6):1172–1176. doi: 10.1021/bi00547a021. [DOI] [PubMed] [Google Scholar]
  5. Jorns M. S., Sancar G. B., Sancar A. Identification of a neutral flavin radical and characterization of a second chromophore in Escherichia coli DNA photolyase. Biochemistry. 1984 Jun 5;23(12):2673–2679. doi: 10.1021/bi00307a021. [DOI] [PubMed] [Google Scholar]
  6. MOROWITZ H. J. Absorption effects in volume irradiation of microorganisms. Science. 1950 Mar 3;111(2879):229–229. doi: 10.1126/science.111.2879.229-a. [DOI] [PubMed] [Google Scholar]
  7. MacQuillan A. M., Herman A., Coberly J. S., Green G. A second photoreactivation-deficient mutation in Saccharomyces cerevisiae. Photochem Photobiol. 1981 Dec;34(6):673–677. [PubMed] [Google Scholar]
  8. Minato S., Werbin H. Spectral properties of the chromophoric material associated with the deoxyribonucleic acid photoreactivating enzyme isolated from baker's yeast. Biochemistry. 1971 Nov 23;10(24):4503–4508. doi: 10.1021/bi00800a025. [DOI] [PubMed] [Google Scholar]
  9. Resnick M. A. A photoreactivationless mutant of Saccharomyces cerevisiae. Photochem Photobiol. 1969 Apr;9(4):307–312. doi: 10.1111/j.1751-1097.1969.tb07294.x. [DOI] [PubMed] [Google Scholar]
  10. Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sancar A., Rupert C. S. Cloning of the phr gene and amplification of photolyase in Escherichia coli. Gene. 1978 Dec;4(4):295–308. doi: 10.1016/0378-1119(78)90047-1. [DOI] [PubMed] [Google Scholar]
  12. Sancar A., Sancar G. B. Escherichia coli DNA photolyase is a flavoprotein. J Mol Biol. 1984 Jan 15;172(2):223–227. doi: 10.1016/s0022-2836(84)80040-6. [DOI] [PubMed] [Google Scholar]
  13. Sancar A., Smith F. W., Sancar G. B. Purification of Escherichia coli DNA photolyase. J Biol Chem. 1984 May 10;259(9):6028–6032. [PubMed] [Google Scholar]
  14. Sancar G. B., Smith F. W., Sancar A. Identification and amplification of the E. coli phr gene product. Nucleic Acids Res. 1983 Oct 11;11(19):6667–6678. doi: 10.1093/nar/11.19.6667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schild D., Johnston J., Chang C., Mortimer R. K. Cloning and mapping of Saccharomyces cerevisiae photoreactivation gene PHR1. Mol Cell Biol. 1984 Sep;4(9):1864–1870. doi: 10.1128/mcb.4.9.1864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Struhl K., Cameron J. R., Davis R. W. Functional genetic expression of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1976 May;73(5):1471–1475. doi: 10.1073/pnas.73.5.1471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Struhl K., Davis R. W. A physical, genetic and transcriptional map of the cloned his3 gene region of Saccharomyces cerevisiae. J Mol Biol. 1980 Jan 25;136(3):309–332. doi: 10.1016/0022-2836(80)90376-9. [DOI] [PubMed] [Google Scholar]

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