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. 1985 Feb;161(2):786–788. doi: 10.1128/jb.161.2.786-788.1985

Efficient transformation of Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.

J C Hinton, M C Perombelon, G P Salmond
PMCID: PMC214956  PMID: 3968041

Abstract

We used a modified version of the method of Hanahan (D. Hanahan, J. Mol. Biol. 166:557-580, 1983) to transform Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica with the plasmids pBR322, pBR325, and pAT153. The transformation frequency ranged from 1 X 10(2) to 4 X 10(4) colonies per micrograms of plasmid DNA. The nature of these transformants was confirmed by plasmid analysis. ColE1-based plasmids make potentially useful cloning vectors for the study of genes involved in the pathogenesis of this species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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