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. 1985 Feb;161(2):799–802. doi: 10.1128/jb.161.2.799-802.1985

Thioredoxin is the bacterial protein encoded by fip that is required for filamentous bacteriophage f1 assembly.

C J Lim, B Haller, J A Fuchs
PMCID: PMC214960  PMID: 3881414

Abstract

Escherichia coli mutants defective in the fip gene contain reduced levels of thioredoxin activity. F' derivatives of trxA mutants failed to support f1 growth, although suppressors of an amber trxA mutation restored both thioredoxin activity and the Fip+ phenotype. Plasmids carrying the trxA gene restored the Fip+ phenotype to fip strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler S., Modrich P. T7-induced DNA polymerase. Requirement for thioredoxin sulfhydryl groups. J Biol Chem. 1983 Jun 10;258(11):6956–6962. [PubMed] [Google Scholar]
  2. Casadaban M. J., Cohen S. N. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. doi: 10.1016/0022-2836(80)90283-1. [DOI] [PubMed] [Google Scholar]
  3. Chumley F. G., Menzel R., Roth J. R. Hfr formation directed by tn10. Genetics. 1979 Apr;91(4):639–655. doi: 10.1093/genetics/91.4.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fuchs J. Isolation of an Escherichia coli mutant deficient in thioredoxin reductase. J Bacteriol. 1977 Feb;129(2):967–972. doi: 10.1128/jb.129.2.967-972.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gonzalez Porqué P., Baldesten A., Reichard P. The involvement of the thioredoxin system in the reduction of methionine sulfoxide and sulfate. J Biol Chem. 1970 May 10;245(9):2371–2374. [PubMed] [Google Scholar]
  6. Holmgren A., Kallis G. B., Nordström B. A mutant thioredoxin from Escherichia coli tsnC 7007 that is nonfunctional as subunit of phage T7 DNA polymerase. J Biol Chem. 1981 Mar 25;256(6):3118–3124. [PubMed] [Google Scholar]
  7. Holmgren A., Ohlsson I., Grankvist M. L. Thiroedoxin from Escherichia coli. Radioimmunological and enzymatic determinations in wild type cells and mutants defective in phage T7 DNA replication. J Biol Chem. 1978 Jan 25;253(2):430–436. [PubMed] [Google Scholar]
  8. Holmgren A., Ohlsson I., Grankvist M. L. Thiroedoxin from Escherichia coli. Radioimmunological and enzymatic determinations in wild type cells and mutants defective in phage T7 DNA replication. J Biol Chem. 1978 Jan 25;253(2):430–436. [PubMed] [Google Scholar]
  9. LAURENT T. C., MOORE E. C., REICHARD P. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEOTIDES. IV. ISOLATION AND CHARACTERIZATION OF THIOREDOXIN, THE HYDROGEN DONOR FROM ESCHERICHIA COLI B. J Biol Chem. 1964 Oct;239:3436–3444. [PubMed] [Google Scholar]
  10. Lunn C. A., Pigiet V. P. Localization of thioredoxin from Escherichia coli in an osmotically sensitive compartment. J Biol Chem. 1982 Oct 10;257(19):11424–11430. [PubMed] [Google Scholar]
  11. Mark D. F., Chase J. W., Richardson C. C. Genetic mapping of trxA, a gene affecting thioredoxin in Escherichia coli K12. Mol Gen Genet. 1977 Oct 20;155(2):145–152. doi: 10.1007/BF00393153. [DOI] [PubMed] [Google Scholar]
  12. Mark D. F., Richardson C. C. Escherichia coli thioredoxin: a subunit of bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1976 Mar;73(3):780–784. doi: 10.1073/pnas.73.3.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nygren H., Rozell B., Holmgren A., Hansson H. A. Immunoelectron microscopic localization of glutaredoxin and thioredoxin in Escherichia coli cells. FEBS Lett. 1981 Oct 12;133(1):145–150. doi: 10.1016/0014-5793(81)80492-9. [DOI] [PubMed] [Google Scholar]
  14. Russel M., Model P. A bacterial gene, fip, required for filamentous bacteriophage fl assembly. J Bacteriol. 1983 Jun;154(3):1064–1076. doi: 10.1128/jb.154.3.1064-1076.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Russel M., Model P. Characterization of the cloned fip gene and its product. J Bacteriol. 1984 Feb;157(2):526–532. doi: 10.1128/jb.157.2.526-532.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Russel M., Model P. Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid. J Bacteriol. 1984 Sep;159(3):1034–1039. doi: 10.1128/jb.159.3.1034-1039.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schaus N., O'Day K., Peters W., Wright A. Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12. J Bacteriol. 1981 Feb;145(2):904–913. doi: 10.1128/jb.145.2.904-913.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sclafani R. A., Wechsler J. A. dnaB125, a dnaB nonsense mutation. J Bacteriol. 1981 Jun;146(3):1170–1173. doi: 10.1128/jb.146.3.1170-1173.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shaw K. J., Berg C. M. Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5. Genetics. 1979 Jul;92(3):741–747. doi: 10.1093/genetics/92.3.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tsang M. L., Schiff J. A. Sulfate-reducing pathway in Escherichia coli involving bound intermediates. J Bacteriol. 1976 Mar;125(3):923–933. doi: 10.1128/jb.125.3.923-933.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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