Abstract
Evidence for the participation of the glutamine transaminase-omega-amidase pathway in the utilization of glutamine in Neurospora crassa was obtained. Its participation is indicated by the in vitro activities of glutamine transaminase and omega-amidase, the in vivo accumulation of alpha-ketoglutaramate when an inhibitor of transamidases is present, and the inhibition by aminooxyacetic acid and 6-diazo-5-oxo-L-norleucine of the ammonium excreted in the presence of glutamine by a mutant strain that lacks glutamate dehydrogenase and glutamate synthase.
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- Cooper A. J., Dhar A. K., Kutt H., Duffy T. E. Determination of 2-pyrrolidone-5-carboxylic and alpha-ketoglutaramic acids in human cerebrospinal fluid by gas chromatography. Anal Biochem. 1980 Mar 15;103(1):118–126. doi: 10.1016/0003-2697(80)90245-6. [DOI] [PubMed] [Google Scholar]
- Cooper A. J., Meister A. The glutamine transaminase-omega-amidase pathway. CRC Crit Rev Biochem. 1977;4(3):281–303. doi: 10.3109/10409237709102560. [DOI] [PubMed] [Google Scholar]
- Cooper A. J. Purification of soluble and mitochondrial glutamine transaminase K from rat kidney. Use of a sensitive assay involving transamination between L-phenylalanine and alpha-keto-gamma-methiolbutyrate. Anal Biochem. 1978 Sep;89(2):451–460. doi: 10.1016/0003-2697(78)90374-3. [DOI] [PubMed] [Google Scholar]
- Dávila G., Sánchez F., Palacios R., Mora J. Genetics and physiology of Neurospora crassa glutamine auxotrophs. J Bacteriol. 1978 Jun;134(3):693–698. doi: 10.1128/jb.134.3.693-698.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Espín G., Palacios R., Mora J. Glutamine metabolism in nitrogen-starved conidia of Neurospora crassa. J Gen Microbiol. 1979 Nov;115(1):59–68. doi: 10.1099/00221287-115-1-59. [DOI] [PubMed] [Google Scholar]
- Hernández G., Sánchez-Pescador R., Palacios R., Mora J. Nitrogen source regulates glutamate dehydrogenase NADP synthesis in Neurospora crassa. J Bacteriol. 1983 Apr;154(1):524–528. doi: 10.1128/jb.154.1.524-528.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hummelt G., Mora J. NADH-dependent glutamate synthase and nitrogen metabolism in Neurospora crassa. Biochem Biophys Res Commun. 1980 Jan 15;92(1):127–133. doi: 10.1016/0006-291x(80)91529-6. [DOI] [PubMed] [Google Scholar]
- Hummelt G., Mora J. Regulation and function of glutamate synthase in Neurospora crassa. Biochem Biophys Res Commun. 1980 Oct 31;96(4):1688–1694. doi: 10.1016/0006-291x(80)91368-6. [DOI] [PubMed] [Google Scholar]
- Imada A., Igarasi S., Nakahama K., Isono M. Asparaginase and glutaminase activities of micro-organisms. J Gen Microbiol. 1973 May;76(1):85–99. doi: 10.1099/00221287-76-1-85. [DOI] [PubMed] [Google Scholar]
- MONDER C., MEISTER A. alpha-Ketoglutaramic acid as a product of enzymic transamination of glutamine in Neurospora. Biochim Biophys Acta. 1958 Apr;28(1):202–203. doi: 10.1016/0006-3002(58)90450-5. [DOI] [PubMed] [Google Scholar]
- Marzluf G. A. Regulation of nitrogen metabolism and gene expression in fungi. Microbiol Rev. 1981 Sep;45(3):437–461. doi: 10.1128/mr.45.3.437-461.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vaca G., Mora J. Nitrogen regulation of arginase in Neurospora crassa. J Bacteriol. 1977 Sep;131(3):719–725. doi: 10.1128/jb.131.3.719-725.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vichido I., Mora Y., Quinto C., Palacios R., Mora J. Nitrogen regulation of glutamine synthetase in Neurospora crassa. J Gen Microbiol. 1978 Jun;106(2):251–259. doi: 10.1099/00221287-106-2-251. [DOI] [PubMed] [Google Scholar]