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. 1985 Mar;161(3):1176–1181. doi: 10.1128/jb.161.3.1176-1181.1985

Exocellular esterase and emulsan release from the cell surface of Acinetobacter calcoaceticus.

Y Shabtai, D L Gutnick
PMCID: PMC215023  PMID: 3838301

Abstract

An esterase activity has been found, both in the cell-free growth medium and on the cell surface of the hydrocarbon-degrading Acinetobacter calcoaceticus RAG-1. The enzyme catalyzed the hydrolysis of acetyl and other acyl groups from triglycerides and aryl and alkyl esters. Emulsan, the extracellular heteropolysaccharide bioemulsifier produced by strain RAG-1, was also a substrate for the enzyme. Gel filtration showed that the cell-free enzyme was released from the cell surface either emulsan free or associated with the bioemulsifier. The partially purified enzyme was found to interact specifically with the esterified fully active emulsan, but not with the deesterified polymer. A role for esterase in emulsan release from the cell surface was indicated when the enzyme was preferentially depleted from the cell surface under conditions in which emulsan was not released. Such cells lost the capacity to release the biopolymer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belsky I., Gutnick D. L., Rosenberg E. Emulsifier of Arthrobacter RAG-1: determination of emulsifier-bound fatty acids. FEBS Lett. 1979 May 1;101(1):175–178. doi: 10.1016/0014-5793(79)81320-4. [DOI] [PubMed] [Google Scholar]
  2. DISCHE Z. New color reactions for determination of sugars in polysaccharides. Methods Biochem Anal. 1955;2:313–358. doi: 10.1002/9780470110188.ch11. [DOI] [PubMed] [Google Scholar]
  3. Goldman S., Shabtai Y., Rubinovitz C., Rosenberg E., Gutnick D. L. Emulsan in Acinetobacter calcoaceticus RAG-1: Distribution of Cell-Free and Cell-Associated Cross-Reacting Material. Appl Environ Microbiol. 1982 Jul;44(1):165–170. doi: 10.1128/aem.44.1.165-170.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gutnick D. L., Rosenberg E. Oil tankers and pollution: a microbiological approach. Annu Rev Microbiol. 1977;31:379–396. doi: 10.1146/annurev.mi.31.100177.002115. [DOI] [PubMed] [Google Scholar]
  5. Krisch K. Reaction of a microsomal esterase from hog-liver with diethyl rho-nitrophenyl phosphate. Biochim Biophys Acta. 1966 Aug 10;122(2):265–280. doi: 10.1016/0926-6593(66)90067-1. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Pines O., Bayer E. A., Gutnick D. L. Localization of emulsan-like polymers associated with the cell surface of acinetobacter calcoaceticus. J Bacteriol. 1983 May;154(2):893–905. doi: 10.1128/jb.154.2.893-905.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pines O., Gutnick D. Specific binding of a bacteriophage at a hydrocarbon-water interface. J Bacteriol. 1984 Jan;157(1):179–183. doi: 10.1128/jb.157.1.179-183.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Reisfeld A., Rosenberg E., Gutnick D. Microbial degradation of crude oil: factors affecting the dispersion in sea water by mixed and pure cultures. Appl Microbiol. 1972 Sep;24(3):363–368. doi: 10.1128/am.24.3.363-368.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rosenberg E., Perry A., Gibson D. T., Gutnick D. L. Emulsifier of Arthrobacter RAG-1: specificity of hydrocarbon substrate. Appl Environ Microbiol. 1979 Mar;37(3):409–413. doi: 10.1128/aem.37.3.409-413.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rosenberg E., Zuckerberg A., Rubinovitz C., Gutnick D. L. Emulsifier of Arthrobacter RAG-1: isolation and emulsifying properties. Appl Environ Microbiol. 1979 Mar;37(3):402–408. doi: 10.1128/aem.37.3.402-408.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rothfield L., Pearlman-Kothencz M. Synthesis and assembly of bacterial membrane components. A lipopolysaccharide-phospholipid-protein complex excreted by living bacteria. J Mol Biol. 1969 Sep 28;44(3):477–492. doi: 10.1016/0022-2836(69)90374-x. [DOI] [PubMed] [Google Scholar]
  13. Rubinovitz C., Gutnick D. L., Rosenberg E. Emulsan production by Acinetobacter calcoaceticus in the presence of chloramphenicol. J Bacteriol. 1982 Oct;152(1):126–132. doi: 10.1128/jb.152.1.126-132.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Winkler U. K., Stuckmann M. Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens. J Bacteriol. 1979 Jun;138(3):663–670. doi: 10.1128/jb.138.3.663-670.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zuckerberg A., Diver A., Peeri Z., Gutnick D. L., Rosenberg E. Emulsifier of Arthrobacter RAG-1: chemical and physical properties. Appl Environ Microbiol. 1979 Mar;37(3):414–420. doi: 10.1128/aem.37.3.414-420.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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