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. 1983 Oct;156(1):186–191. doi: 10.1128/jb.156.1.186-191.1983

Protein synthesis by intact Coxiella burnetii cells.

R L Zuerner, H A Thompson
PMCID: PMC215068  PMID: 6194147

Abstract

Coxiella burnetii was isolated from persistently infected fibroblast host cells by a rapid mechanical lysis technique. Macromolecular synthesis was initiated in these otherwise dormant cells by incubation at pH 4.5. The synthesis of protein proceeded for as long as 24 h. Initiation of protein synthesis in C. burnetii was dependent upon RNA synthesis. Approximately 24 species of polypeptides were synthesized, and some of these appeared to be major synthetic products. Increases in protein biomass of 15 to 30% were calculated to occur during incubation. Inhibition of DNA synthesis affected protein synthesis after 12 h of incubation. The results suggest that although these parasitic bacteria did not grow in the axenic media devised, significant biosynthetic processes occurred.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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