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. 1983 Oct;156(1):257–263. doi: 10.1128/jb.156.1.257-263.1983

Genes controlling xylan utilization by Bacillus subtilis.

M I Roncero
PMCID: PMC215078  PMID: 6413490

Abstract

Eight mutants of Bacillus subtilis deficient in xylan utilization were isolated and characterized genetically and biochemically. Each mutant was obtained independently after nitrosoguanidine mutagenesis. All of the analyzed mutations were shown to be linked. Reciprocal transformation crosses revealed the existence of two genes controlling xylan utilization which have been designated xynA and xynB. Available data have indicated that these two genes code for two xylan-degrading enzymes existing in the wild-type strains, an extracellular beta-xylanase (xynA) and a cell-associated beta-xylosidase (xynB).

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Selected References

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  1. Biely P., Vrsanská M., Krátký Z. Xylan-degrading enzymes of the yeast Cryptococcus albidus. Identification and cellular localization. Eur J Biochem. 1980;108(1):313–321. doi: 10.1111/j.1432-1033.1980.tb04725.x. [DOI] [PubMed] [Google Scholar]
  2. Boylan R. J., Mendelson N. H., Brooks D., Young F. E. Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid. J Bacteriol. 1972 Apr;110(1):281–290. doi: 10.1128/jb.110.1.281-290.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carlton B. C. Fine-structure mapping by transformation in the tryptophan region of Bacillus subtilis. J Bacteriol. 1966 May;91(5):1795–1803. doi: 10.1128/jb.91.5.1795-1803.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gryczan T. J., Contente S., Dubnau D. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J Bacteriol. 1978 Apr;134(1):318–329. doi: 10.1128/jb.134.1.318-329.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Han Y. W., Srinivasan V. R. Purification and characterization of beta-glucosidase of Alcaligenes faecalis. J Bacteriol. 1969 Dec;100(3):1355–1363. doi: 10.1128/jb.100.3.1355-1363.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Henner D. J., Hoch J. A. The Bacillus subtilis chromosome. Microbiol Rev. 1980 Mar;44(1):57–82. doi: 10.1128/mr.44.1.57-82.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. IVANOVICS G., CSISZAR K. Isolation and some characteristics of subtilis pages with transdducing activity. Acta Microbiol Acad Sci Hung. 1962;9:209–218. [PubMed] [Google Scholar]
  8. LACKS S., HOTCHKISS R. D. A study of the genetic material determining an enzyme in Pneumococcus. Biochim Biophys Acta. 1960 Apr 22;39:508–518. doi: 10.1016/0006-3002(60)90205-5. [DOI] [PubMed] [Google Scholar]
  9. Lepesant-Kejzlarová J., Lepesant J. A., Walle J., Billault A., Dedonder R. Revision of the linkage map of Bacillus subtilis 168: indications for circularity of the chromosome. J Bacteriol. 1975 Mar;121(3):823–834. doi: 10.1128/jb.121.3.823-834.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Notario V., Villa T. G., Villanueva J. R. beta-Xylosidases in the yeast Cryptococcus albidus var. aerius. Can J Microbiol. 1976 Feb;22(2):312–315. doi: 10.1139/m76-044. [DOI] [PubMed] [Google Scholar]
  11. Ruiz-Vázquez R., Pueyo C., Cerdá-Olmedo E. A mutagen assay detecting forward mutations in an arabinose-sensitive strain of Salmonella typhimurium. Mutat Res. 1978 Oct;54(2):121–129. doi: 10.1016/0165-1161(78)90032-8. [DOI] [PubMed] [Google Scholar]
  12. Sekiguchi J., Takada N., Okada H. Genes affecting the productivity of alpha-amylase in Bacillus subtilis Marburg. J Bacteriol. 1975 Feb;121(2):688–694. doi: 10.1128/jb.121.2.688-694.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Steinmetz M., Kunst F., Dedonder R. Mapping of mutations affecting synthesis of exocellular enzymes in Bacillus subtilis. Identity of the sacUh, amyB and pap mutations. Mol Gen Genet. 1976 Nov 17;148(3):281–285. doi: 10.1007/BF00332902. [DOI] [PubMed] [Google Scholar]
  15. TAKAHASHI I. Transducing phages for Bacillus subtilis. J Gen Microbiol. 1963 May;31:211–217. doi: 10.1099/00221287-31-2-211. [DOI] [PubMed] [Google Scholar]
  16. Yamaguchi K., Nagata Y., Maruo B. Genetic control of the rate of alpha-amylase synthesis in Bacillus subtilis. J Bacteriol. 1974 Aug;119(2):410–415. doi: 10.1128/jb.119.2.410-415.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yamaguchi K., Nagata Y., Maruo B. Isolation of mutants defective in alpha-amylase from Bacillus subtilis: genetic analyses. J Bacteriol. 1974 Aug;119(2):416–424. doi: 10.1128/jb.119.2.416-424.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yasbin R. E., Wilson G. A., Young F. E. Transformation and transfection in lysogenic strains of Bacillus subtilis 168. J Bacteriol. 1973 Feb;113(2):540–548. doi: 10.1128/jb.113.2.540-548.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yoneda Y., Maruo B. Mutation of Bacillus subtilis causing hyperproduction of alpha-amylase and protease, and its synergistic effect. J Bacteriol. 1975 Oct;124(1):48–54. doi: 10.1128/jb.124.1.48-54.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Young F. E., Smith C., Reilly B. E. Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis. J Bacteriol. 1969 Jun;98(3):1087–1097. doi: 10.1128/jb.98.3.1087-1097.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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