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. 1983 Oct;156(1):301–307. doi: 10.1128/jb.156.1.301-307.1983

Carbon catabolite repression of maltase synthesis in Saccharomyces carlsbergensis.

H J Federoff, T R Eccleshall, J Marmur
PMCID: PMC215083  PMID: 6352680

Abstract

Carbon catabolite repression of maltase gene expression is brought about by the addition of glucose, resulting in a drastic inhibition of the induction of maltase. When added to induced cells, glucose leads to the inhibition of maltase synthesis within 30 min, which can be accounted for by the disappearance of hybridizable maltase RNA sequences. The loss of maltase-specific RNA due to catabolite repression can be traced to the combined effects of a 15-fold decrease in the rate of transcription of the maltase structural gene 15 to 20 min after the addition of glucose and a change in the half-life of maltase mRNA. However, the stability of maltase, once induced, is not affected by the addition of glucose.

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Selected References

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  1. Beier D. R., Young E. T. Characterization of a regulatory region upstream of the ADR2 locus of S. cerevisiae. Nature. 1982 Dec 23;300(5894):724–728. doi: 10.1038/300724a0. [DOI] [PubMed] [Google Scholar]
  2. Carlson M., Botstein D. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase. Cell. 1982 Jan;28(1):145–154. doi: 10.1016/0092-8674(82)90384-1. [DOI] [PubMed] [Google Scholar]
  3. Elorza M. V., Lostau C. M., Villanueva J. R., Sentandreu R. Invertase messenger ribonucleic acid in Saccharomyces cerevisiae. Kinetics of formation and decay. Biochim Biophys Acta. 1977 Apr 19;475(4):638–651. doi: 10.1016/0005-2787(77)90324-0. [DOI] [PubMed] [Google Scholar]
  4. Entian K. D. Genetic and biochemical evidence for hexokinase PII as a key enzyme involved in carbon catabolite repression in yeast. Mol Gen Genet. 1980;178(3):633–637. doi: 10.1007/BF00337871. [DOI] [PubMed] [Google Scholar]
  5. Entian K. D., Zimmermann F. K. Glycolytic enzymes and intermediates in carbon catabolite repression mutants of Saccharomyces cerevisiae. Mol Gen Genet. 1980 Jan;177(2):345–350. doi: 10.1007/BF00267449. [DOI] [PubMed] [Google Scholar]
  6. Federoff H. J., Cohen J. D., Eccleshall T. R., Needleman R. B., Buchferer B. A., Giacalone J., Marmur J. Isolation of a maltase structural gene from Saccharomyces carlsbergensis. J Bacteriol. 1982 Mar;149(3):1064–1070. doi: 10.1128/jb.149.3.1064-1070.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Federoff H. J., Eccleshall T. R., Marmur J. Regulation of maltase synthesis in Saccharomyces carlsbergensis. J Bacteriol. 1983 Jun;154(3):1301–1308. doi: 10.1128/jb.154.3.1301-1308.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fried H. M., Warner J. R. Cloning of yeast gene for trichodermin resistance and ribosomal protein L3. Proc Natl Acad Sci U S A. 1981 Jan;78(1):238–242. doi: 10.1073/pnas.78.1.238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Guyette W. A., Matusik R. J., Rosen J. M. Prolactin-mediated transcriptional and post-transcriptional control of casein gene expression. Cell. 1979 Aug;17(4):1013–1023. doi: 10.1016/0092-8674(79)90340-4. [DOI] [PubMed] [Google Scholar]
  10. Görts C. P. Effect of glucose on the activity and the kinetics of the maltose-uptake system and of alpha-glucosidase in Saccharomyces cerevisiae. Biochim Biophys Acta. 1969 Jul 30;184(2):299–305. doi: 10.1016/0304-4165(69)90032-4. [DOI] [PubMed] [Google Scholar]
  11. Kim C. H., Warner J. R. Messenger RNA for ribosomal proteins in yeast. J Mol Biol. 1983 Mar 25;165(1):79–89. doi: 10.1016/s0022-2836(83)80243-5. [DOI] [PubMed] [Google Scholar]
  12. Maitra P. K., Lobo Z. A kinetic study of glycolytic enzyme synthesis in yeast. J Biol Chem. 1971 Jan 25;246(2):475–488. [PubMed] [Google Scholar]
  13. Mazón M. J., Gancedo J. M., Gancedo C. Phosphorylation and inactivation of yeast fructose-bisphosphatase in vivo by glucose and by proton ionophores. A possible role for cAMP. Eur J Biochem. 1982 Oct;127(3):605–608. doi: 10.1111/j.1432-1033.1982.tb06915.x. [DOI] [PubMed] [Google Scholar]
  14. McAlister L., Holland M. J. Targeted deletion of a yeast enolase structural gene. Identification and isolation of yeast enolase isozymes. J Biol Chem. 1982 Jun 25;257(12):7181–7188. [PubMed] [Google Scholar]
  15. Michels C. A., Romanowski A. Pleiotropic glucose repression-resistant mutation in Saccharomyces carlesbergensis. J Bacteriol. 1980 Aug;143(2):674–679. doi: 10.1128/jb.143.2.674-679.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mormeneo S., Sentandreu R. Regulation of invertase synthesis by glucose in Saccharomyces cerevisiae. J Bacteriol. 1982 Oct;152(1):14–18. doi: 10.1128/jb.152.1.14-18.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Needleman R. B., Michels C. Repeated family of genes controlling maltose fermentation in Saccharomyces carlsbergensis. Mol Cell Biol. 1983 May;3(5):796–802. doi: 10.1128/mcb.3.5.796. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pearson N. J., Fried H. M., Warner J. R. Yeast use translational control to compensate for extra copies of a ribosomal protein gene. Cell. 1982 Jun;29(2):347–355. doi: 10.1016/0092-8674(82)90151-9. [DOI] [PubMed] [Google Scholar]
  19. Perlman D., Halvorson H. O. Distinct repressible mRNAs for cytoplasmic and secreted yeast invertase are encoded by a single gene. Cell. 1981 Aug;25(2):525–536. doi: 10.1016/0092-8674(81)90071-4. [DOI] [PubMed] [Google Scholar]
  20. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  21. Wiskocil R., Bensky P., Dower W., Goldberger R. F., Gordon J. I., Deeley R. G. Coordinate regulation of two estrogen-dependent genes in avian liver. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4474–4478. doi: 10.1073/pnas.77.8.4474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zitomer R. S., Montgomery D. L., Nichols D. L., Hall B. D. Transcriptional regulation of the yeast cytochrome c gene. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3627–3631. doi: 10.1073/pnas.76.8.3627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. de Kroon R. A., Koningsberger V. V. An inducible transport system for alpha-glucosides in protoplasts of Saccharomyces carlsbergensis. Biochim Biophys Acta. 1970 Apr 15;204(2):590–609. doi: 10.1016/0005-2787(70)90178-4. [DOI] [PubMed] [Google Scholar]
  24. ten Berge A. M., Zoutewelle G., van de Poll K. W. Regulation of maltose fermentation in Saccharomyces carlsbergensis. I. The function of the gene MAL6, as recognized by mal6-mutants. Mol Gen Genet. 1973 Jul 2;123(3):233–246. doi: 10.1007/BF00271242. [DOI] [PubMed] [Google Scholar]

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