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. 1983 Oct;156(1):316–326. doi: 10.1128/jb.156.1.316-326.1983

Biosynthetic pathways in Methanospirillum hungatei as determined by 13C nuclear magnetic resonance.

I Ekiel, I C Smith, G D Sprott
PMCID: PMC215085  PMID: 6619097

Abstract

The main metabolic pathways in Methanospirillum hungatei GP1 were followed by using 13C nuclear magnetic resonance, with 13C-labeled acetate and CO2 as carbon sources. The labeling patterns found in carbohydrates, amino acids, lipids, and nucleosides were consistent with the formation of pyruvate from acetate and CO2 as the first step in biosynthesis. Carbohydrates are formed by the glucogenic pathway, and no scrambling of label was observed, indicating that the oxidative or reductive pentose phosphate pathways are not functioning at significant rates. The pathways for amino acid biosynthesis are the usual ones, with the exception of that for isoleucine. The tricarboxylic acid pathway is incomplete and operates in a reductive direction to form alpha-ketoglutarate. The phytanyl chains of lipids are synthesized from acetate via mevalonic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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