Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Jan;157(1):28–34. doi: 10.1128/jb.157.1.28-34.1984

Properties of a Streptococcus lactis strain that ferments lactose slowly.

V L Crow, T D Thomas
PMCID: PMC215124  PMID: 6418719

Abstract

Streptococcus lactis 7962, which ferments lactose slowly, has a lactose phosphoenolpyruvate-dependent phosphotransferase system and low phospho-beta-galactosidase activity, in addition to high beta-galactosidase activity. Lactose 6'-phosphate accumulated to a high concentration (greater than 100 mM) in cells growing on lactose. In contrast, lactic streptococci, which ferment lactose rapidly and use only the lactose-phosphotransferase system for uptake, contained high phospho-beta-galactosidase activity and low concentrations (0.9 to 1.6 mM) of lactose 6'-phosphate. It is concluded that rate-limiting phospho-beta-galactosidase activity is primarily responsible for defective lactose metabolism in S. lactis 7962.

Full text

PDF
28

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bissett D. L., Anderson R. L. Lactose and D-galactose metabolism in group N streptococci: presence of enzymes for both the D-galactose 1-phosphate and D-tagatose 6-phosphate pathways. J Bacteriol. 1974 Jan;117(1):318–320. doi: 10.1128/jb.117.1.318-320.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bissett D. L., Wenger W. C., Anderson R. L. Lactose and D-galactose metabolism in Staphylococcus aureus. II. Isomerization of D-galactose 6-phosphate to D-tagatose 6-phosphate by a specific D-galactose-6-phosphate isomerase. J Biol Chem. 1980 Sep 25;255(18):8740–8744. [PubMed] [Google Scholar]
  3. Crow V. L., Davey G. P., Pearce L. E., Thomas T. D. Plasmid linkage of the D-tagatose 6-phosphate pathway in Streptococcus lactis: effect on lactose and galactose metabolism. J Bacteriol. 1983 Jan;153(1):76–83. doi: 10.1128/jb.153.1.76-83.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dills S. S., Apperson A., Schmidt M. R., Saier M. H., Jr Carbohydrate transport in bacteria. Microbiol Rev. 1980 Sep;44(3):385–418. doi: 10.1128/mr.44.3.385-418.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Farrow J. A. Lactose hydrolysing enzymes in Streptococcus lactis and Streptococcus cremoris and also in some other species of streptococci. J Appl Bacteriol. 1980 Dec;49(3):493–503. doi: 10.1111/j.1365-2672.1980.tb04724.x. [DOI] [PubMed] [Google Scholar]
  6. Fraenkel D. G. The accumulation of glucose 6-phosphate from glucose and its effect in an Escherichia coli mutant lacking phosphoglucose isomerase and glucose 6-phosphate dehydrogenase. J Biol Chem. 1968 Dec 25;243(24):6451–6457. [PubMed] [Google Scholar]
  7. Hartree E. F. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem. 1972 Aug;48(2):422–427. doi: 10.1016/0003-2697(72)90094-2. [DOI] [PubMed] [Google Scholar]
  8. Johnson K. G., McDonald I. J. Beta-D-phosphogalactoside galactohydrolase from Streptococcus cremoris HP: purification and enzyme properties. J Bacteriol. 1974 Feb;117(2):667–674. doi: 10.1128/jb.117.2.667-674.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kashket E. R., Wilson T. H. Role of metabolic energy in the transport of -galactosides by Streptococcus lactis. J Bacteriol. 1972 Feb;109(2):784–789. doi: 10.1128/jb.109.2.784-789.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McKay L. L., Walter L. A., Sandine W. E., Elliker P. R. Involvement of phosphoenolpyruvate in lactose utilization by group N streptococci. J Bacteriol. 1969 Aug;99(2):603–610. doi: 10.1128/jb.99.2.603-610.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McKay L., Miller A., 3rd, Sandine W. E., Elliker P. R. Mechanisms of lactose utilization by lactic acid streptococci: enzymatic and genetic analyses. J Bacteriol. 1970 Jun;102(3):804–809. doi: 10.1128/jb.102.3.804-809.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Molskness T. A., Lee D. R., Sandine W. E., Elliker P. R. -D-phosphogalactoside galactohydrolase of lactic streptococci. Appl Microbiol. 1973 Mar;25(3):373–380. doi: 10.1128/am.25.3.373-380.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Postma P. W., Roseman S. The bacterial phosphoenolpyruvate: sugar phosphotransferase system. Biochim Biophys Acta. 1976 Dec 14;457(3-4):213–257. doi: 10.1016/0304-4157(76)90001-0. [DOI] [PubMed] [Google Scholar]
  14. Rahn O., Hegarty C. P., Deuel R. E. FACTORS INFLUENCING THE RATE OF FERMENTATION OF STREPTOCOCCUS LACTIS. J Bacteriol. 1938 May;35(5):547–558. doi: 10.1128/jb.35.5.547-558.1938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thomas T. D., Ellwood D. C., Longyear V. M. Change from homo- to heterolactic fermentation by Streptococcus lactis resulting from glucose limitation in anaerobic chemostat cultures. J Bacteriol. 1979 Apr;138(1):109–117. doi: 10.1128/jb.138.1.109-117.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Thomas T. D., Jarvis B. D., Skipper N. A. Localization of proteinase(s) near the cell surface of Streptococcus lactis. J Bacteriol. 1974 May;118(2):329–333. doi: 10.1128/jb.118.2.329-333.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Thomas T. D. Regulation of lactose fermentation in group N streptococci. Appl Environ Microbiol. 1976 Oct;32(4):474–478. doi: 10.1128/aem.32.4.474-478.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Thomas T. D., Turner K. W., Crow V. L. Galactose fermentation by Streptococcus lactis and Streptococcus cremoris: pathways, products, and regulation. J Bacteriol. 1980 Nov;144(2):672–682. doi: 10.1128/jb.144.2.672-682.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Thompson J., Chassy B. M. Novel phosphoenolpyruvate-dependent futile cycle in Streptococcus lactis: 2-deoxy-D-glucose uncouples energy production from growth. J Bacteriol. 1982 Sep;151(3):1454–1465. doi: 10.1128/jb.151.3.1454-1465.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Thompson J. Lactose metabolism in Streptococcus lactis: phosphorylation of galactose and glucose moieties in vivo. J Bacteriol. 1979 Dec;140(3):774–785. doi: 10.1128/jb.140.3.774-785.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES