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. 1998;77(1):98–102. doi: 10.1038/bjc.1998.15

Expression and degeneration of tenascin-C in human lung cancers.

H Kusagawa 1, K Onoda 1, S Namikawa 1, I Yada 1, A Okada 1, T Yoshida 1, T Sakakura 1
PMCID: PMC2151247  PMID: 9459152

Abstract

Tenascin-C is an extracellular matrix glycoprotein produced in response to epithelial-mesenchymal interactions during organogenesis and tissue remodelling. It has therefore been proposed as a stromal marker for epithelial malignancy. To test this hypothesis, 30 human lung cancers, presenting a variety of clinicopathological features, and six specimens of normal tissue were examined by Western and Northern blotting of tenascin-C protein and mRNA. The results obtained were: (1) elevated tenascin-C expression was detected in all 30 cases by Western blotting, with mRNA increase in 22 of them; (2) mRNA for a large isoform of tenascin-C, including an alternatively spliced sequence, was expressed in lung cancer tissues but not in normal lungs; and (3) metastasis to lymph nodes was frequently found in cases whose tenascin-C was degraded into small fragments. These results suggest that tenascin-C degradation can be used as a marker for metastatic potential of a tumour.

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Selected References

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