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. 1998;77(1):11–14. doi: 10.1038/bjc.1998.2

Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia.

J Ramsay 1, G Birrell 1, K Baumann 1, A Bodero 1, P Parsons 1, M Lavin 1
PMCID: PMC2151277  PMID: 9459139

Abstract

The human melanoma cell lines MM96L, A2058 and HT144 were examined for sensitivity to ionizing radiation and UVB radiation. HT144 demonstrated a significant increase in sensitivity to ionizing and UVB radiation compared with the MM96L and A2058 cells. Sensitivity to both agents was associated with susceptibility to apoptosis. Using a protein truncation assay, a mutation for the gene for ataxia telangiectasia (ATM) was identified in HT144 cells. This was confirmed to be a homozygous mutation by subsequent sequencing of the abnormal region. Protein truncation assay of the other two cell lines showed no abnormality. The results suggest that somatic mutation of the A-T gene may be important in determining tumour radiosensitivity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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