Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1998;77(3):391–395. doi: 10.1038/bjc.1998.62

Characterization of native fluorescence from DMBA-treated hamster cheek pouch buccal mucosa for measuring tissue transformation.

N Vengadesan 1, P Aruna 1, S Ganesan 1
PMCID: PMC2151305  PMID: 9472633

Abstract

The steady-state native fluorescence spectra of extracts of normal mucosa as well as of different stages of oral lesion of the 7,12-dimethylbenz (a) anthracene (DMBA)-induced hamster cheek pouch carcinogenesis model have been measured and analysed at 405 nm excitation. The emission spectra were scanned from 430 to 700 nm to characterize the native fluorescence of endogenous porphyrin and other fluorophores under various tissue transformation conditions, such as hyperplasia, papilloma, early invasive carcinoma and well-differentiated squamous cell carcinoma. Two ratio parameters, R1 = (I530/I620) and R2 = (I530/I630), are introduced to quantify the diagnostic potentiality. The ratio values were found to decrease as the stage of the cancer increases. The suggested critical values for both R1 and R2 for normal mucosa is above three, and the suggested critical value for tissues with lesion is less than three. It was also found that the values for R1 and R2 for well-differentiated squamous cell carcinoma is less than one. The ratio parameter R2 is selected for discrimination between normal mucosa and oral lesions, as the difference in the R2 value between normal and DMBA-treated tissue is higher than that for R1.

Full text

PDF
391

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alfano R. R., Das B. B., Cleary J., Prudente R., Celmer E. J. Light sheds light on cancer--distinguishing malignant tumors from benign tissues and tumors. Bull N Y Acad Med. 1991 Mar-Apr;67(2):143–150. [PMC free article] [PubMed] [Google Scholar]
  2. Alfano R. R., Yao S. S. Human teeth with and without dental caries studied by visible luminescent spectroscopy. J Dent Res. 1981 Feb;60(2):120–122. doi: 10.1177/00220345810600020401. [DOI] [PubMed] [Google Scholar]
  3. Andersson-Engels S., Johansson J., Svanberg K., Svanberg S. Fluorescence imaging and point measurements of tissue: applications to the demarcation of malignant tumors and atherosclerotic lesions from normal tissue. Photochem Photobiol. 1991 Jun;53(6):807–814. doi: 10.1111/j.1751-1097.1991.tb09895.x. [DOI] [PubMed] [Google Scholar]
  4. Baumgartner R., Fisslinger H., Jocham D., Lenz H., Ruprecht L., Stepp H., Unsöld E. A fluorescence imaging device for endoscopic detection of early stage cancer--instrumental and experimental studies. Photochem Photobiol. 1987 Nov;46(5):759–763. doi: 10.1111/j.1751-1097.1987.tb04844.x. [DOI] [PubMed] [Google Scholar]
  5. Boring C. C., Squires T. S., Tong T. Cancer statistics, 1991. CA Cancer J Clin. 1991 Jan-Feb;41(1):19–36. doi: 10.3322/canjclin.41.1.19. [DOI] [PubMed] [Google Scholar]
  6. Braichotte D. R., Wagnières G. A., Bays R., Monnier P., van den Bergh H. E. Clinical pharmacokinetic studies of photofrin by fluorescence spectroscopy in the oral cavity, the esophagus, and the bronchi. Cancer. 1995 Jun 1;75(11):2768–2778. doi: 10.1002/1097-0142(19950601)75:11<2768::aid-cncr2820751122>3.0.co;2-i. [DOI] [PubMed] [Google Scholar]
  7. CHANCE B., BALTSCHEFFSKY H. Respiratory enzymes in oxidative phosphorylation. VII. Binding of intramitochondrial reduced pyridine nucleotide. J Biol Chem. 1958 Sep;233(3):736–739. [PubMed] [Google Scholar]
  8. Crean D. H., Liebow C., Penetrante R. B., Mang T. S. Evaluation of porfimer sodium fluorescence for measuring tissue transformation. Cancer. 1993 Nov 15;72(10):3068–3077. doi: 10.1002/1097-0142(19931115)72:10<3068::aid-cncr2820721032>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  9. GHADIALLY F. N., NEISH W. J., DAWKINS H. C. Mechanisms involved in the production of red fluorescence of human and experimental tumours. J Pathol Bacteriol. 1963 Jan;85:77–92. doi: 10.1002/path.1700850108. [DOI] [PubMed] [Google Scholar]
  10. GHADIALLY F. N., NEISH W. J. Porphyrin fluorescence of experimentally produced squamous cell carcinoma. Nature. 1960 Dec 24;188:1124–1124. doi: 10.1038/1881124a0. [DOI] [PubMed] [Google Scholar]
  11. Harris D. M., Werkhaven J. Endogenous porphyrin fluorescence in tumors. Lasers Surg Med. 1987;7(6):467–472. doi: 10.1002/lsm.1900070605. [DOI] [PubMed] [Google Scholar]
  12. Kapadia C. R., Cutruzzola F. W., O'Brien K. M., Stetz M. L., Enriquez R., Deckelbaum L. I. Laser-induced fluorescence spectroscopy of human colonic mucosa. Detection of adenomatous transformation. Gastroenterology. 1990 Jul;99(1):150–157. doi: 10.1016/0016-5085(90)91242-x. [DOI] [PubMed] [Google Scholar]
  13. MORRIS A. L. Factors influencing experimental carcinogensis in the hamster cheek pouch. J Dent Res. 1961 Jan-Feb;40:3–15. doi: 10.1177/00220345610400012001. [DOI] [PubMed] [Google Scholar]
  14. Pathak I., Davis N. L., Hsiang Y. N., Quenville N. F., Palcic B. Detection of squamous neoplasia by fluorescence imaging comparing porfimer sodium fluorescence to tissue autofluorescence in the hamster cheek-pouch model. Am J Surg. 1995 Nov;170(5):423–426. doi: 10.1016/s0002-9610(99)80321-3. [DOI] [PubMed] [Google Scholar]
  15. SALLEY J. J. Experimental carcinogenesis in the cheek pouch of the Syrian hamster. J Dent Res. 1954 Apr;33(2):253–262. doi: 10.1177/00220345540330021201. [DOI] [PubMed] [Google Scholar]
  16. Schomacker K. T., Frisoli J. K., Compton C. C., Flotte T. J., Richter J. M., Nishioka N. S., Deutsch T. F. Ultraviolet laser-induced fluorescence of colonic tissue: basic biology and diagnostic potential. Lasers Surg Med. 1992;12(1):63–78. doi: 10.1002/lsm.1900120111. [DOI] [PubMed] [Google Scholar]
  17. Shklar G., Eisenberg E., Flynn E. Immunoenhancing agents and experimental leukoplakia and carcinoma of the hamster buccal pouch. Prog Exp Tumor Res. 1979;24:269–282. doi: 10.1159/000402104. [DOI] [PubMed] [Google Scholar]
  18. Urade M., Uematsu T., Mima T., Ogura T., Matsuya T. Serum dipeptidyl peptidase (DPP) IV activity in hamster buccal pouch carcinogenesis with 9,10-dimethyl-1,2-benzanthracene. J Oral Pathol Med. 1992 Mar;21(3):109–112. doi: 10.1111/j.1600-0714.1992.tb00992.x. [DOI] [PubMed] [Google Scholar]
  19. Yang Y. L., Ye Y. M., Li F. M., Li Y. F., Ma P. Z. Characteristic autofluorescence for cancer diagnosis and its origin. Lasers Surg Med. 1987;7(6):528–532. doi: 10.1002/lsm.1900070617. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES