Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1986 Jun;166(3):713–721. doi: 10.1128/jb.166.3.713-721.1986

Nucleotide sequence of the surface exclusion genes traS and traT from the IncF0 lac plasmid pED208.

B B Finlay, W Paranchych
PMCID: PMC215180  PMID: 3011738

Abstract

pED208 is a 90-kilobase conjugative plasmid belonging to the incompatibility group IncF0 lac. The surface exclusion system from this plasmid was cloned and sequenced, and two genes demonstrated exclusion ability. traS encoded a 186-amino-acid hydrophobic protein which, when transcribed from a vector promoter, caused exclusion of pED208. The product of traT (TraTp) was a 245-residue protein which was highly expressed independently of a vector promoter in Escherichia coli minicells. The TraTp from pED208 was homologous with traT products from the IncF plasmids R-100 and F (80% homology), but recombinants containing the pED208 surface exclusion system excluded F poorly.

Full text

PDF
713

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Kennedy N., Skurray R. Cell--cell interactions in conjugating Escherichia coli: role of traT protein in surface exclusion. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5104–5108. doi: 10.1073/pnas.74.11.5104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Achtman M., Manning P. A., Edelbluth C., Herrlich P. Export without proteolytic processing of inner and outer membrane proteins encoded by F sex factor tra cistrons in Escherichia coli minicells. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4837–4841. doi: 10.1073/pnas.76.10.4837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Agüero M. E., Aron L., DeLuca A. G., Timmis K. N., Cabello F. C. A plasmid-encoded outer membrane protein, TraT, enhances resistance of Escherichia coli to phagocytosis. Infect Immun. 1984 Dec;46(3):740–746. doi: 10.1128/iai.46.3.740-746.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. Phage Folac: an Folac plasmid-dependent bacteriophage. J Gen Microbiol. 1981 Oct;126(2):405–411. doi: 10.1099/00221287-126-2-405. [DOI] [PubMed] [Google Scholar]
  6. Chou P. Y., Fasman G. D. Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978;47:45–148. doi: 10.1002/9780470122921.ch2. [DOI] [PubMed] [Google Scholar]
  7. Falkow S., Baron L. S. EPISOMIC ELEMENT IN A STRAIN OF SALMONELLA TYPHOSA. J Bacteriol. 1962 Sep;84(3):581–589. doi: 10.1128/jb.84.3.581-589.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ferrazza D., Levy S. B. Biosynthesis of a plasmid-encoded outer membrane surface exclusion protein involves processing from a precursor polypeptide. J Biol Chem. 1980 Oct 10;255(19):8955–8958. [PubMed] [Google Scholar]
  9. Finlay B. B., Frost L. S., Paranchych W. Localization, cloning, and sequence determination of the conjugative plasmid ColB2 pilin gene. J Bacteriol. 1984 Oct;160(1):402–407. doi: 10.1128/jb.160.1.402-407.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Finlay B. B., Paranchych W., Falkow S. Characterization of conjugative plasmid EDP208. J Bacteriol. 1983 Oct;156(1):230–235. doi: 10.1128/jb.156.1.230-235.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Frost L. S., Armstrong G. D., Finlay B. B., Edwards B. F., Paranchych W. N-terminal amino acid sequencing of EDP208 conjugative pili. J Bacteriol. 1983 Feb;153(2):950–954. doi: 10.1128/jb.153.2.950-954.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Frost L. S., Finlay B. B., Opgenorth A., Paranchych W., Lee J. S. Characterization and sequence analysis of pilin from F-like plasmids. J Bacteriol. 1985 Dec;164(3):1238–1247. doi: 10.1128/jb.164.3.1238-1247.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Frost L. S., Paranchych W., Willetts N. S. DNA sequence of the F traALE region that includes the gene for F pilin. J Bacteriol. 1984 Oct;160(1):395–401. doi: 10.1128/jb.160.1.395-401.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hawley D. K., McClure W. R. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983 Apr 25;11(8):2237–2255. doi: 10.1093/nar/11.8.2237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hopp T. P., Woods K. R. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824–3828. doi: 10.1073/pnas.78.6.3824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Johnson D. A., Willetts N. S. Construction and characterization of multicopy plasmids containing the entire F transfer region. Plasmid. 1980 Nov;4(3):292–304. doi: 10.1016/0147-619x(80)90068-2. [DOI] [PubMed] [Google Scholar]
  17. Kennedy N., Beutin L., Achtman M., Skurray R., Rahmsdorf U., Herrlich P. Conjugation proteins encoded by the F sex factor. Nature. 1977 Dec 15;270(5638):580–585. doi: 10.1038/270580a0. [DOI] [PubMed] [Google Scholar]
  18. Lederberg J, Cavalli L L, Lederberg E M. Sex Compatibility in Escherichia Coli. Genetics. 1952 Nov;37(6):720–730. doi: 10.1093/genetics/37.6.720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Minkley E. G., Jr, Ippen-Ihler K. Identification of a membrane protein associated with expression of the surface exclusion region of the F transfer operon. J Bacteriol. 1977 Mar;129(3):1613–1622. doi: 10.1128/jb.129.3.1613-1622.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Minkley E. G., Jr, Willetts N. S. Overproduction, purification and characterization of the F traT protein. Mol Gen Genet. 1984;196(2):225–235. doi: 10.1007/BF00328054. [DOI] [PubMed] [Google Scholar]
  21. Moll A., Manning P. A., Timmis K. N. Plasmid-determined resistance to serum bactericidal activity: a major outer membrane protein, the traT gene product, is responsible for plasmid-specified serum resistance in Escherichia coli. Infect Immun. 1980 May;28(2):359–367. doi: 10.1128/iai.28.2.359-367.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ogata R. T., Winters C., Levine R. P. Nucleotide sequence analysis of the complement resistance gene from plasmid R100. J Bacteriol. 1982 Aug;151(2):819–827. doi: 10.1128/jb.151.2.819-827.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Perumal N. B., Minkley E. G., Jr The product of the F sex factor traT surface exclusion gene is a lipoprotein. J Biol Chem. 1984 May 10;259(9):5357–5360. [PubMed] [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Scott J. R. Regulation of plasmid replication. Microbiol Rev. 1984 Mar;48(1):1–23. doi: 10.1016/b978-0-12-048850-6.50006-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shine J., Dalgarno L. Determinant of cistron specificity in bacterial ribosomes. Nature. 1975 Mar 6;254(5495):34–38. doi: 10.1038/254034a0. [DOI] [PubMed] [Google Scholar]
  27. Skurray R. A., Nagaishi H., Clark A. J. Molecular cloning of DNA from F sex factor of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1976 Jan;73(1):64–68. doi: 10.1073/pnas.73.1.64. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  29. Willetts N., Maule J. Characterisation of a lambda transducing phage carrying the F conjugation gene traG. Mol Gen Genet. 1980;178(3):675–680. doi: 10.1007/BF00337878. [DOI] [PubMed] [Google Scholar]
  30. Willetts N., Skurray R. The conjugation system of F-like plasmids. Annu Rev Genet. 1980;14:41–76. doi: 10.1146/annurev.ge.14.120180.000353. [DOI] [PubMed] [Google Scholar]
  31. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES