Fig. 5.

PCR analysis of genomic DNA from different cell lines with primer pairs each specific for Chinese or Syrian (Golden) hamster. Primer: (a) act-cHam-F and act-cHam-R; (b) glo-sHam-F and glo-sHam-R. Amplified DNA fragments were detected after ethidium bromide staining of 1.2% agarose gels. Lane 1, BHK-21 (ACC 61); lane 2, CHO-K1 (ACC 110); lane 3, CHO (ACC -); lane 4, HAP-T1 (ACC 222); lane 5, HKT-1097 (ACC 445); lane 6, HPD-1NR (ACC 314); lane 7, HPD-2NR (ACC 293); lane 8, M3E3/C3 (ACC 340); lane 9, V-79 (ACC 335); lane 10, CHO-DHFR (ACC 126); lane 11, PC-12 (ACC 159); lane 12, NIH-3T3 (ACC 59); lane 13, water control (no DNA). C, Chinese hamster; M, Mouse; R, Rat; S, Syrian hamster. The arrows indicate the DNA molecular weight markers of 500 and 1,200 bp