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. Author manuscript; available in PMC: 2008 Jun 6.
Published in final edited form as: Neuron. 2007 Dec 6;56(5):823–837. doi: 10.1016/j.neuron.2007.09.035

Figure 5. CASK interactions with presynaptic proteins are regulated by Cdk5.

Figure 5

(a) Membrane fractions were prepared from E17 wild-type or littermate Cdk5 deficient brains and subjected to Neurexin IPs. (b) Membrane fractions from E17 wild-type or littermate Cdk5 deficient brains were subjected to Veli or Mint1 IPs. (c) Membrane fractions were prepared from E17 wild-type (lanes 1–2) or littermate Cdk5 deficient (lanes 3–4) brains. Fractions were lysed in DOC buffer, dialyzed against .1% Triton buffer and subjected to IP with an α1B (lanes 2 & 4) or control (lanes 1 & 3) antibody. (d) Membrane fractions were prepared from two week induced p25 transgenic mice or wild-type littermate brains. Fractions were subsequently lysed in DOC buffer, dialyzed against .1% Triton buffer and subjected to IP with an α1B (lanes 3–4) or control (lanes 1–2) antibody.