Abstract
A complementation test was devised for the fungus Cryptococcus neoformans. Complementation was signalled by the growth of prototrophic heterokaryons generated in crosses of the type aB X Ab, where a and b represent any two of the genetic markers ilv1, cys1, cys2, and cys3. The cloned complementing heterokaryons formed characteristic hyphal colonies that contained both hyphae and yeast cells. The heterokaryon-derived yeasts were of three kinds: parental haploids, recombinant haploids, and diploids.
Full text
PDF![924](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/245f/215214/8bb01330d53b/jbacter00211-0236.png)
![925](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/245f/215214/7408e034e535/jbacter00211-0237.png)
![926](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/245f/215214/39d72d4bf1d4/jbacter00211-0238.png)
![927](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/245f/215214/bf3bceca3736/jbacter00211-0239.png)
![928](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/245f/215214/e2a77e0efe2c/jbacter00211-0240.png)
![929](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/245f/215214/fb8f8bf76e3d/jbacter00211-0241.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Kwon-Chung K. J. A new genus, filobasidiella, the perfect state of Cryptococcus neoformans. Mycologia. 1975 Nov-Dec;67(6):1197–1200. [PubMed] [Google Scholar]
- Kwon-Chung K. J. Morphogenesis of Filobasidiella neoformans, the sexual state of Cryptococcus neoformans. Mycologia. 1976 Jul-Aug;68(4):821–833. [PubMed] [Google Scholar]
- Kwon-Chung K. J. Nuclear genotypes of spore chains in Filobasidiella neoformans (Cryptococcus neoformans). Mycologia. 1980 Mar-Apr;72(2):418–422. [PubMed] [Google Scholar]
- Still C. N., Jacobson E. S. Recombinational mapping of capsule mutations in Cryptococcus neoformans. J Bacteriol. 1983 Oct;156(1):460–462. doi: 10.1128/jb.156.1.460-462.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White C. W., Jacobson E. S. Occurrence of diploid strains of Cryptococcus neoformans. J Bacteriol. 1985 Mar;161(3):1231–1232. doi: 10.1128/jb.161.3.1231-1232.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]